GC-MS studies of thiophenes in the supercritical fluid CO2 and solvent extracts of tagetes patula L.

Szabolcs Szarka, István Gyurján, Miklós László, Éva Héthelyi, Inna N. Kuzovkina, E. Lemberkovics, E. Szőke

Research output: Article

3 Citations (Scopus)

Abstract

The intact plant parts and genetically modified hairy root clone #TpA6 of Tagetes patula were extracted with supercritical fluid CO2 extraction (SFE) and a conventional solvent extraction. SFE optimization included the variation of fluid CO2 pressure, dynamic time, and the addition of methanol modifier co-solvent. The four characteristic thiophene metabolites, 5-(3-buten-1-ynyl)-2,2′-bithienyl (BBT), 2,2′:5′,2″- terthiophene (α-T), 5-(4-acetoxy-1-butynyl)-2,2′-bithienyl (BBTOAc), and 5-(3,4-diacetoxy-1-butynyl)-2,2′-bithienyl [BBT(OAc)2], were analysed by GC-MS. The proposed SFE method allowed the selective extraction of thiophenes in 60 min dynamic time with supercritical CO2 without modifier co-solvent, at 30 MPa and 40 °C. The SFE and the reference solvent extraction yielded similar results. The SFE of intact roots and flowers yielded 717 ± 31.3 and 480 ± 26.6 μg g-1 α-T, respectively, while the leaves did not contain considerable amounts of thiophenes. Remarkable amounts of BBT, BBTOAc, and BBT(OAc)2 were characteristic of the SFE of hairy root cultures.

Original languageEnglish
Pages (from-to)1039-1047
Number of pages9
JournalChromatographia
Volume71
Issue number11-12
DOIs
Publication statusPublished - 2010

Fingerprint

Tagetes
Supercritical Fluid Chromatography
Thiophenes
Supercritical fluids
Solvent extraction
Genetically Modified Plants
Methanol
Metabolites
Clone Cells
Pressure
Fluids

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

Cite this

GC-MS studies of thiophenes in the supercritical fluid CO2 and solvent extracts of tagetes patula L. / Szarka, Szabolcs; Gyurján, István; László, Miklós; Héthelyi, Éva; Kuzovkina, Inna N.; Lemberkovics, E.; Szőke, E.

In: Chromatographia, Vol. 71, No. 11-12, 2010, p. 1039-1047.

Research output: Article

Szarka, Szabolcs ; Gyurján, István ; László, Miklós ; Héthelyi, Éva ; Kuzovkina, Inna N. ; Lemberkovics, E. ; Szőke, E. / GC-MS studies of thiophenes in the supercritical fluid CO2 and solvent extracts of tagetes patula L. In: Chromatographia. 2010 ; Vol. 71, No. 11-12. pp. 1039-1047.
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abstract = "The intact plant parts and genetically modified hairy root clone #TpA6 of Tagetes patula were extracted with supercritical fluid CO2 extraction (SFE) and a conventional solvent extraction. SFE optimization included the variation of fluid CO2 pressure, dynamic time, and the addition of methanol modifier co-solvent. The four characteristic thiophene metabolites, 5-(3-buten-1-ynyl)-2,2′-bithienyl (BBT), 2,2′:5′,2″- terthiophene (α-T), 5-(4-acetoxy-1-butynyl)-2,2′-bithienyl (BBTOAc), and 5-(3,4-diacetoxy-1-butynyl)-2,2′-bithienyl [BBT(OAc)2], were analysed by GC-MS. The proposed SFE method allowed the selective extraction of thiophenes in 60 min dynamic time with supercritical CO2 without modifier co-solvent, at 30 MPa and 40 °C. The SFE and the reference solvent extraction yielded similar results. The SFE of intact roots and flowers yielded 717 ± 31.3 and 480 ± 26.6 μg g-1 α-T, respectively, while the leaves did not contain considerable amounts of thiophenes. Remarkable amounts of BBT, BBTOAc, and BBT(OAc)2 were characteristic of the SFE of hairy root cultures.",
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AU - Szarka, Szabolcs

AU - Gyurján, István

AU - László, Miklós

AU - Héthelyi, Éva

AU - Kuzovkina, Inna N.

AU - Lemberkovics, E.

AU - Szőke, E.

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AB - The intact plant parts and genetically modified hairy root clone #TpA6 of Tagetes patula were extracted with supercritical fluid CO2 extraction (SFE) and a conventional solvent extraction. SFE optimization included the variation of fluid CO2 pressure, dynamic time, and the addition of methanol modifier co-solvent. The four characteristic thiophene metabolites, 5-(3-buten-1-ynyl)-2,2′-bithienyl (BBT), 2,2′:5′,2″- terthiophene (α-T), 5-(4-acetoxy-1-butynyl)-2,2′-bithienyl (BBTOAc), and 5-(3,4-diacetoxy-1-butynyl)-2,2′-bithienyl [BBT(OAc)2], were analysed by GC-MS. The proposed SFE method allowed the selective extraction of thiophenes in 60 min dynamic time with supercritical CO2 without modifier co-solvent, at 30 MPa and 40 °C. The SFE and the reference solvent extraction yielded similar results. The SFE of intact roots and flowers yielded 717 ± 31.3 and 480 ± 26.6 μg g-1 α-T, respectively, while the leaves did not contain considerable amounts of thiophenes. Remarkable amounts of BBT, BBTOAc, and BBT(OAc)2 were characteristic of the SFE of hairy root cultures.

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