Galanin-immunoreactive axons innervate somatostatin-synthesizing neurons in the anterior periventricular nucleus of the rat

Z. Liposits, István Merchenthaler, JoAnne J. Reid, Andres Negro-Vilar

Research output: Article

27 Citations (Scopus)

Abstract

The anterior periventricular nucleus (PeN) regulates GH secretion by synthesizing and releasing somatotropin release-inhibiting factor (SRIF) into the portal circulation. This territory of the diencephalon is heavily innervated by axons of galanin (GAL)-immunoreactive (IR) neurons. The connections between GAL-IR fibers and hypophysiotropic SRIF neurons were studied by means of immunocytochemical double labeling at the light and electron microscopic levels. Retrograde axonal labeling with Fluoro-Gold revealed the anterior PeN as the main site of hypophysiotropic SRIF-synthesizing neurons. These cells were densely surrounded by GALergic axons that made contacts with their cell bodies and dendrites. At the ultrastructural level, diaminobenzidine-labeled SRIF neurons received synapsing GAL-IR axons marked with silver-gold particles. Both axo-somatic and axo-dendritic forms of connections were observed. These morphochemical data revealed an interaction between GAL-and SRIF-synthesizing neurons in the anterior PeN. Furthermore, the presence of synaptic connections between these neuronal systems suggests a role for GAL in the control of SRIF secretion and, in turn, in the regulation of GH release.

Original languageEnglish
Pages (from-to)917-923
Number of pages7
JournalEndocrinology
Volume132
Issue number2
Publication statusPublished - febr. 1993

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Galanin
Somatostatin
Axons
Neurons
Diencephalon
Dendrites
Silver
Gold
Electrons
Light

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Galanin-immunoreactive axons innervate somatostatin-synthesizing neurons in the anterior periventricular nucleus of the rat. / Liposits, Z.; Merchenthaler, István; Reid, JoAnne J.; Negro-Vilar, Andres.

In: Endocrinology, Vol. 132, No. 2, 02.1993, p. 917-923.

Research output: Article

Liposits, Z. ; Merchenthaler, István ; Reid, JoAnne J. ; Negro-Vilar, Andres. / Galanin-immunoreactive axons innervate somatostatin-synthesizing neurons in the anterior periventricular nucleus of the rat. In: Endocrinology. 1993 ; Vol. 132, No. 2. pp. 917-923.
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