Functional importance of neuronal nitric oxide synthase in the endothelium of rat basilar arteries

Zoltán Benyó, Z. Lacza, T. Hortobágyi, Christoph Görlach, Michael Wahl

Research output: Article

18 Citations (Scopus)

Abstract

The function of the neuronal isoform of nitric oxide synthase (nNOS) was studied by comparing the effects of the specific nNOS blocker 7-nitro indazole monosodium salt (7-NINA) with that of the general NOS inhibitor N(G)-nitro-L-arginine (L-NA) in isolated rat basilar arteries (BAs). 7-NINA had no significant effect on the resting tone of the vessels, while both L-NA and 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ), a selective inhibitor of the soluble guanylyl cyclase, induced contraction. The relaxant effect of bradykinin was attenuated in the presence of L-NA but was not changed by 7- NINA. In contrast, 7-NINA markedly reduced the acetylcholine-induced, endothelium-dependent relaxation. These results demonstrate that nNOS contributes significantly to the relaxant effect of acetylcholine, indicating the functional importance of this isoenzyme in the cerebrovascular endothelium. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)79-84
Number of pages6
JournalBrain Research
Volume877
Issue number1
DOIs
Publication statusPublished - szept. 15 2000

Fingerprint

Nitric Oxide Synthase Type I
Basilar Artery
Endothelium
Protein Isoforms
Salts
Acetylcholine
Bradykinin
Isoenzymes
Arginine
7-nitroindazole

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Functional importance of neuronal nitric oxide synthase in the endothelium of rat basilar arteries. / Benyó, Zoltán; Lacza, Z.; Hortobágyi, T.; Görlach, Christoph; Wahl, Michael.

In: Brain Research, Vol. 877, No. 1, 15.09.2000, p. 79-84.

Research output: Article

Benyó, Zoltán ; Lacza, Z. ; Hortobágyi, T. ; Görlach, Christoph ; Wahl, Michael. / Functional importance of neuronal nitric oxide synthase in the endothelium of rat basilar arteries. In: Brain Research. 2000 ; Vol. 877, No. 1. pp. 79-84.
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