Forensic interlaboratory evaluation of the ForFLUID kit for vaginal fluids identification

Saverio Giampaoli, Federica Alessandrini, Andrea Berti, Luigi Ripani, Ajin Choi, Roselien Crab, Elisabetta De Vittori, Balazs Egyed, Cordula Haas, Hwan Young Lee, Marie Korabecná, Fabrice Noel, Daniele Podini, Adriano Tagliabracci, Alessio Valentini, Vincenzo Romano Spica

Research output: Article

14 Citations (Scopus)


Identification of vaginal fluids is an important step in the process of sexual assaults confirmation. Advances in both microbiology and molecular biology defined technical approaches allowing the discrimination of body fluids. These protocols are based on the identification of specific bacterial communities by microfloraDNA (mfDNA) amplification. A multiplex real time-PCR assay (ForFLUID kit) has been developed for identifying biological fluids and for discrimination among vaginal, oral and fecal samples. In order to test its efficacy and reliability of the assay in the identification of vaginal fluids, an interlaboratory evaluation has been performed on homogeneous vaginal swabs. All the involved laboratories were able to correctly recognize all the vaginal swabs, and no false positives were identified when the assay was applied on non-vaginal samples. The assay represents an useful molecular tool that can be easily adopted by forensic geneticists involved in vaginal fluid identification.

Original languageEnglish
Pages (from-to)60-63
Number of pages4
JournalJournal of Forensic and Legal Medicine
Publication statusPublished - jan. 1 2014

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Law

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    Giampaoli, S., Alessandrini, F., Berti, A., Ripani, L., Choi, A., Crab, R., De Vittori, E., Egyed, B., Haas, C., Lee, H. Y., Korabecná, M., Noel, F., Podini, D., Tagliabracci, A., Valentini, A., & Romano Spica, V. (2014). Forensic interlaboratory evaluation of the ForFLUID kit for vaginal fluids identification. Journal of Forensic and Legal Medicine, 21, 60-63.