Fluorescent derivatives of bovine neurotensin 8-13 fragment

Péter Kele, Eszter Illyés, Gábor Mezö, Margit Dóda, Marianna Mák, András D. Kaposi, Ferenc Sebestyén

Research output: Article

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Abstract

Fluorescent derivatives of bovine neurotensin 8-13 fragment were prepared. For N-terminal labelling, 4-[7-hydroxycoumaryl]acetic acid (Hca), 4-[7-methoxycoumaryl]acetic acid (Mca) and 2-amino-3-[4-[7-methoxycoumaryl]]propionic acid (Amp) were used while the C-terminus of the peptide chain was elongated with Amp. The fluorescence excitation and emission spectra of the peptide derivatives were studied. Hca- and Mca/Amp-derivatives were easily distinguishable because of the 60 nm shift of their emission maxima. Compared with the natural sequence, the presence of an N-terminal label did not influence the biological potency in a longitudinal muscle strip of guinea-pig ileum, while labelling at the C-terminus considerably reduced the activity of the peptide.

Original languageEnglish
Pages (from-to)235-238
Number of pages4
JournalInternational Journal of Peptide Research and Therapeutics
Volume6
Issue number4
DOIs
Publication statusPublished - jan. 1 1999

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ASJC Scopus subject areas

  • Analytical Chemistry
  • Bioengineering
  • Biochemistry
  • Molecular Medicine
  • Drug Discovery

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