Flavonoid aglycones can compete with Ochratoxin A for human serum albumin: A new possible mode of action

Miklós Poór, S. Kunsági-Máté, Tímea Bencsik, József Petrik, Sanda Vladimir-Knežević, T. Kőszegi

Research output: Article

22 Citations (Scopus)

Abstract

The mycotoxin Ochratoxin A (OTA) appears worldwide in cereals, plant products, different foods and drinks. Ochratoxin A binds to plasma albumin with a very high affinity. However, it is well known that natural flavonoids can also bind to human serum albumin (HSA) at the same binding site as OTA does (site I, subdomain IIA). A few experimental literature data suggest that reducing the bound fraction of OTA speeds up its elimination rate with a potential decrease in its toxicity. In our experimental model competitive binding properties of flavonoid aglycones were examined with a fluorescence polarization based approach. Our data show that some of the flavonoids are able to remove the toxin from HSA. We conclude that among the 13 studied flavonoid aglycones galangin and quercetin were the most effective competitors for OTA.

Original languageEnglish
Pages (from-to)279-283
Number of pages5
JournalInternational Journal of Biological Macromolecules
Volume51
Issue number3
DOIs
Publication statusPublished - okt. 2012

Fingerprint

Flavonoids
Serum Albumin
Food products plants
Fluorescence Polarization
Competitive Binding
Mycotoxins
Quercetin
Toxicity
Theoretical Models
Fluorescence
Binding Sites
ochratoxin A
Polarization
Food

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Structural Biology

Cite this

Flavonoid aglycones can compete with Ochratoxin A for human serum albumin : A new possible mode of action. / Poór, Miklós; Kunsági-Máté, S.; Bencsik, Tímea; Petrik, József; Vladimir-Knežević, Sanda; Kőszegi, T.

In: International Journal of Biological Macromolecules, Vol. 51, No. 3, 10.2012, p. 279-283.

Research output: Article

@article{ef53a0cca33e4dc2b18dc40cdeabbf7b,
title = "Flavonoid aglycones can compete with Ochratoxin A for human serum albumin: A new possible mode of action",
abstract = "The mycotoxin Ochratoxin A (OTA) appears worldwide in cereals, plant products, different foods and drinks. Ochratoxin A binds to plasma albumin with a very high affinity. However, it is well known that natural flavonoids can also bind to human serum albumin (HSA) at the same binding site as OTA does (site I, subdomain IIA). A few experimental literature data suggest that reducing the bound fraction of OTA speeds up its elimination rate with a potential decrease in its toxicity. In our experimental model competitive binding properties of flavonoid aglycones were examined with a fluorescence polarization based approach. Our data show that some of the flavonoids are able to remove the toxin from HSA. We conclude that among the 13 studied flavonoid aglycones galangin and quercetin were the most effective competitors for OTA.",
keywords = "Competitive interaction, Flavonoids, Fluorescence polarization, Human serum albumin, Ochratoxin A",
author = "Mikl{\'o}s Po{\'o}r and S. Kuns{\'a}gi-M{\'a}t{\'e} and T{\'i}mea Bencsik and J{\'o}zsef Petrik and Sanda Vladimir-Knežević and T. Kőszegi",
year = "2012",
month = "10",
doi = "10.1016/j.ijbiomac.2012.05.019",
language = "English",
volume = "51",
pages = "279--283",
journal = "International Journal of Biological Macromolecules",
issn = "0141-8130",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Flavonoid aglycones can compete with Ochratoxin A for human serum albumin

T2 - A new possible mode of action

AU - Poór, Miklós

AU - Kunsági-Máté, S.

AU - Bencsik, Tímea

AU - Petrik, József

AU - Vladimir-Knežević, Sanda

AU - Kőszegi, T.

PY - 2012/10

Y1 - 2012/10

N2 - The mycotoxin Ochratoxin A (OTA) appears worldwide in cereals, plant products, different foods and drinks. Ochratoxin A binds to plasma albumin with a very high affinity. However, it is well known that natural flavonoids can also bind to human serum albumin (HSA) at the same binding site as OTA does (site I, subdomain IIA). A few experimental literature data suggest that reducing the bound fraction of OTA speeds up its elimination rate with a potential decrease in its toxicity. In our experimental model competitive binding properties of flavonoid aglycones were examined with a fluorescence polarization based approach. Our data show that some of the flavonoids are able to remove the toxin from HSA. We conclude that among the 13 studied flavonoid aglycones galangin and quercetin were the most effective competitors for OTA.

AB - The mycotoxin Ochratoxin A (OTA) appears worldwide in cereals, plant products, different foods and drinks. Ochratoxin A binds to plasma albumin with a very high affinity. However, it is well known that natural flavonoids can also bind to human serum albumin (HSA) at the same binding site as OTA does (site I, subdomain IIA). A few experimental literature data suggest that reducing the bound fraction of OTA speeds up its elimination rate with a potential decrease in its toxicity. In our experimental model competitive binding properties of flavonoid aglycones were examined with a fluorescence polarization based approach. Our data show that some of the flavonoids are able to remove the toxin from HSA. We conclude that among the 13 studied flavonoid aglycones galangin and quercetin were the most effective competitors for OTA.

KW - Competitive interaction

KW - Flavonoids

KW - Fluorescence polarization

KW - Human serum albumin

KW - Ochratoxin A

UR - http://www.scopus.com/inward/record.url?scp=84863618882&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84863618882&partnerID=8YFLogxK

U2 - 10.1016/j.ijbiomac.2012.05.019

DO - 10.1016/j.ijbiomac.2012.05.019

M3 - Article

C2 - 22634515

AN - SCOPUS:84863618882

VL - 51

SP - 279

EP - 283

JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

SN - 0141-8130

IS - 3

ER -