Fixation of laccase enzyme into polypyrrole, assisted by chemical interaction with modified magnetite nanoparticles: A facile route to synthesize stable electroactive bionanocomposite catalysts

B. Endrodi, A. Kormányos, C. Janáky, O. Berkesi, C. Visy

Research output: Article

5 Citations (Scopus)

Abstract

Effective bio-electrocatalysts require stable immobilization of sufficient amounts of the bioactive component. In this study, a novel and efficient method for specific binding of laccase enzyme onto magnetite nanoparticles (NPs) is presented. The interaction between the chemically modified magnetite NPs and the enzyme was evidenced by both infrared (FT-IR) and X-ray photoelectron spectroscopy (XPS). Subsequently, the enzyme-coated magnetite NPs were successfully incorporated into polypyrrole (PPy) matrix during galvanostatic electropolymerization. The encapsulation of laccase covered NPs was proved by EQCN, TEM, and FT-IR spectroscopy; whereas the electrochemical behaviour of the formed bionanocomposite was characterized by cyclic voltammetry. In oxygen saturated solution a cathodic charge surplus was observed, related to the electrochemical reduction of oxygen. This surplus was two times higher in the case of the laccase containing layer compared to its only magnetite containing counterpart. Kinetic aspects of the oxygen reduction reaction (ORR) on the laccase containing films were investigated by hydrodynamic voltammetry, and the four-electron route was found to be exclusive, which is promising from the fuel cell perspective. Such synergistic combination of inorganic NPs and enzymes may open new avenues in the application of these bio-nanocomposite materials.

Original languageEnglish
Pages (from-to)282-288
Number of pages7
JournalElectrochimica Acta
Volume122
DOIs
Publication statusPublished - márc. 10 2014

Fingerprint

Magnetite Nanoparticles
Magnetite nanoparticles
Laccase
Polypyrroles
Enzymes
Catalysts
Oxygen
Ferrosoferric Oxide
Nanoparticles
Electropolymerization
Electrocatalysts
Magnetite
Voltammetry
Encapsulation
Cyclic voltammetry
Fuel cells
Infrared spectroscopy
Nanocomposites
Hydrodynamics
X ray photoelectron spectroscopy

ASJC Scopus subject areas

  • Electrochemistry
  • Chemical Engineering(all)

Cite this

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title = "Fixation of laccase enzyme into polypyrrole, assisted by chemical interaction with modified magnetite nanoparticles: A facile route to synthesize stable electroactive bionanocomposite catalysts",
abstract = "Effective bio-electrocatalysts require stable immobilization of sufficient amounts of the bioactive component. In this study, a novel and efficient method for specific binding of laccase enzyme onto magnetite nanoparticles (NPs) is presented. The interaction between the chemically modified magnetite NPs and the enzyme was evidenced by both infrared (FT-IR) and X-ray photoelectron spectroscopy (XPS). Subsequently, the enzyme-coated magnetite NPs were successfully incorporated into polypyrrole (PPy) matrix during galvanostatic electropolymerization. The encapsulation of laccase covered NPs was proved by EQCN, TEM, and FT-IR spectroscopy; whereas the electrochemical behaviour of the formed bionanocomposite was characterized by cyclic voltammetry. In oxygen saturated solution a cathodic charge surplus was observed, related to the electrochemical reduction of oxygen. This surplus was two times higher in the case of the laccase containing layer compared to its only magnetite containing counterpart. Kinetic aspects of the oxygen reduction reaction (ORR) on the laccase containing films were investigated by hydrodynamic voltammetry, and the four-electron route was found to be exclusive, which is promising from the fuel cell perspective. Such synergistic combination of inorganic NPs and enzymes may open new avenues in the application of these bio-nanocomposite materials.",
keywords = "Laccase, Magnetite, Nanocomposite, Oxygen reduction reaction, Polypyrrole",
author = "B. Endrodi and A. Korm{\'a}nyos and C. Jan{\'a}ky and O. Berkesi and C. Visy",
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T1 - Fixation of laccase enzyme into polypyrrole, assisted by chemical interaction with modified magnetite nanoparticles

T2 - A facile route to synthesize stable electroactive bionanocomposite catalysts

AU - Endrodi, B.

AU - Kormányos, A.

AU - Janáky, C.

AU - Berkesi, O.

AU - Visy, C.

PY - 2014/3/10

Y1 - 2014/3/10

N2 - Effective bio-electrocatalysts require stable immobilization of sufficient amounts of the bioactive component. In this study, a novel and efficient method for specific binding of laccase enzyme onto magnetite nanoparticles (NPs) is presented. The interaction between the chemically modified magnetite NPs and the enzyme was evidenced by both infrared (FT-IR) and X-ray photoelectron spectroscopy (XPS). Subsequently, the enzyme-coated magnetite NPs were successfully incorporated into polypyrrole (PPy) matrix during galvanostatic electropolymerization. The encapsulation of laccase covered NPs was proved by EQCN, TEM, and FT-IR spectroscopy; whereas the electrochemical behaviour of the formed bionanocomposite was characterized by cyclic voltammetry. In oxygen saturated solution a cathodic charge surplus was observed, related to the electrochemical reduction of oxygen. This surplus was two times higher in the case of the laccase containing layer compared to its only magnetite containing counterpart. Kinetic aspects of the oxygen reduction reaction (ORR) on the laccase containing films were investigated by hydrodynamic voltammetry, and the four-electron route was found to be exclusive, which is promising from the fuel cell perspective. Such synergistic combination of inorganic NPs and enzymes may open new avenues in the application of these bio-nanocomposite materials.

AB - Effective bio-electrocatalysts require stable immobilization of sufficient amounts of the bioactive component. In this study, a novel and efficient method for specific binding of laccase enzyme onto magnetite nanoparticles (NPs) is presented. The interaction between the chemically modified magnetite NPs and the enzyme was evidenced by both infrared (FT-IR) and X-ray photoelectron spectroscopy (XPS). Subsequently, the enzyme-coated magnetite NPs were successfully incorporated into polypyrrole (PPy) matrix during galvanostatic electropolymerization. The encapsulation of laccase covered NPs was proved by EQCN, TEM, and FT-IR spectroscopy; whereas the electrochemical behaviour of the formed bionanocomposite was characterized by cyclic voltammetry. In oxygen saturated solution a cathodic charge surplus was observed, related to the electrochemical reduction of oxygen. This surplus was two times higher in the case of the laccase containing layer compared to its only magnetite containing counterpart. Kinetic aspects of the oxygen reduction reaction (ORR) on the laccase containing films were investigated by hydrodynamic voltammetry, and the four-electron route was found to be exclusive, which is promising from the fuel cell perspective. Such synergistic combination of inorganic NPs and enzymes may open new avenues in the application of these bio-nanocomposite materials.

KW - Laccase

KW - Magnetite

KW - Nanocomposite

KW - Oxygen reduction reaction

KW - Polypyrrole

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