FISH chromosome mapping allowing karyotype analysis in Medicago truncatula lines Jemalong J5 and R-108-1

Malika Cerbah, Zoltan Kevei, Sonja Siljak-Yakovlev, Eva Kondorosi, Adam Kondorosi, Toan Hanh Trinh

Research output: Article

27 Citations (Scopus)

Abstract

Two lines of Medicago truncatula, R-108-1 and Jemalong J5, exhibit differences in their symbiotic properties, morphological markers, and capacity for regeneration and transformation. Using cytogenetic techniques, we detected differences in chromomycin A3 staining on the two sides of the secondary constriction of the nucleolar chromosome pair of J5 and R-108-1, while DAPI (4', 6-diamidino-2-phenylindole) staining revealed no differences between the two lines. FISH (fluorescent in situ hybridization) analysis showed a difference between J5 and R-108-1 for the same 5S rRNA genes; three loci were found in J5 but only two loci in R-108-1. However, for the 18S-5.8S-26S rDNA genes only a single locus was found in both lines. Based on these analyses, a karyotype for the two lines was established.

Original languageEnglish
Pages (from-to)947-950
Number of pages4
JournalMolecular Plant-Microbe Interactions
Volume12
Issue number11
DOIs
Publication statusPublished - nov. 1999

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ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science

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