First report of Cryptosporidium deer-like genotype in Malaysian cattle

N. Abdul Halim, J. Plutzer, M. A. Bakheit, P. Karanis

Research output: Article

32 Citations (Scopus)


Fifty faecal samples from diarrheic calves between 1 and 6 months old were collected per rectum from 5 farms around Petaling District in Selangor, Malaysia for Cryptosporidium species detection and genotyping investigation. Oocysts were purified using sedimentation and gradient centrifugation, then examined by immunofluorescence assay (IFAT). Genomic DNA was extracted from all samples and nested PCR was performed to amplify the SSU rRNA gene. Eighteen samples (36%) were positive for Cryptosporidium species by PCR. The sequence and phylogenetic analysis of 14 isolates indicated that Cryptosporidium parvum was most common (11 isolates) followed by Cryptosporidium deer-like genotype (3 isolates). The present work reports the first data on Cryptosporidium genotyping from cattle in Malaysia.

Original languageEnglish
Pages (from-to)325-329
Number of pages5
JournalVeterinary Parasitology
Issue number3-4
Publication statusPublished - ápr. 15 2008

ASJC Scopus subject areas

  • Parasitology
  • veterinary(all)

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