Extrachromosomal rDNA of Tetrahymena thermophila is not a perfect palindrome

G. Kiss, Ronald E. Pearlman

Research output: Article

18 Citations (Scopus)

Abstract

We have determined the restriction-endonuclease-cleavage map and the nucleotide sequence of the central 1.4 kb fragment of the macronuclear extrachromosomal rDNA of Tetrahymena thermophila. These data demonstrate that this molecule is not a perfect palindrome, having a 29 bp AT-rich non-palindromic sequence at its center. This observation is important in determining the mechanism by which a single chromosomally integrated rRNA gene in the micronucleus is rearranged and amplified during sexual development to yield multiple copies of extrachromosomal rDNA in the macronucleus.

Original languageEnglish
Pages (from-to)281-287
Number of pages7
JournalGene
Volume13
Issue number3
DOIs
Publication statusPublished - 1981

Fingerprint

Tetrahymena thermophila
Ribosomal DNA
Macronucleus
Sexual Development
DNA Restriction Enzymes
rRNA Genes

ASJC Scopus subject areas

  • Genetics

Cite this

Extrachromosomal rDNA of Tetrahymena thermophila is not a perfect palindrome. / Kiss, G.; Pearlman, Ronald E.

In: Gene, Vol. 13, No. 3, 1981, p. 281-287.

Research output: Article

Kiss, G. ; Pearlman, Ronald E. / Extrachromosomal rDNA of Tetrahymena thermophila is not a perfect palindrome. In: Gene. 1981 ; Vol. 13, No. 3. pp. 281-287.
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KW - Ribosomal RNA gene

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