Extracellular cell wall β(1,3)glucan is required to couple septation to actomyosin ring contraction

Javier Muñoz, Juan Carlos G Cortés, M. Sipiczki, Mariona Ramos, José Angel Clemente-Ramos, M. Belén Moreno, Ivone M. Martins, Pilar Pérez, Juan Carlos Ribas

Research output: Article

38 Citations (Scopus)

Abstract

Cytokinesis has been extensively studied in different models, but the role of the extracellular cell wall is less understood. Here we studied this process in fission yeast. The essential protein Bgs4 synthesizes the main cell wall β(1,3)glucan. We show that Bgs4-derived β(1,3)glucan is required for correct and stable actomyosin ring positioning in the cell middle, before the start of septum formation and anchorage to the cell wall. Consequently, β(1,3)glucan loss generated ring sliding, oblique positioned rings and septa, misdirected septum synthesis indicative of relaxed rings, and uncoupling between a fast ring and membrane ingression and slow septum synthesis, suggesting that cytokinesis can progress with defective septum pushing and/or ring pulling forces. Moreover, Bgs4-derived β(1,3)glucan is essential for secondary septum formation and correct primary septum completion. Therefore, our results show that extracellular β(1,3)glucan is required for cytokinesis to connect the cell wall with the plasma membrane and for contractile ring function, as proposed for the equivalent extracellular matrix in animal cells.

Original languageEnglish
Pages (from-to)265-282
Number of pages18
JournalJournal of Cell Biology
Volume203
Issue number2
DOIs
Publication statusPublished - okt. 28 2013

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Actomyosin
Glucans
Cell Wall
Cytokinesis
Schizosaccharomyces
Extracellular Matrix
Cell Membrane
Membranes
Proteins

ASJC Scopus subject areas

  • Cell Biology

Cite this

Muñoz, J., Cortés, J. C. G., Sipiczki, M., Ramos, M., Clemente-Ramos, J. A., Moreno, M. B., ... Ribas, J. C. (2013). Extracellular cell wall β(1,3)glucan is required to couple septation to actomyosin ring contraction. Journal of Cell Biology, 203(2), 265-282. https://doi.org/10.1083/jcb.201304132

Extracellular cell wall β(1,3)glucan is required to couple septation to actomyosin ring contraction. / Muñoz, Javier; Cortés, Juan Carlos G; Sipiczki, M.; Ramos, Mariona; Clemente-Ramos, José Angel; Moreno, M. Belén; Martins, Ivone M.; Pérez, Pilar; Ribas, Juan Carlos.

In: Journal of Cell Biology, Vol. 203, No. 2, 28.10.2013, p. 265-282.

Research output: Article

Muñoz, J, Cortés, JCG, Sipiczki, M, Ramos, M, Clemente-Ramos, JA, Moreno, MB, Martins, IM, Pérez, P & Ribas, JC 2013, 'Extracellular cell wall β(1,3)glucan is required to couple septation to actomyosin ring contraction', Journal of Cell Biology, vol. 203, no. 2, pp. 265-282. https://doi.org/10.1083/jcb.201304132
Muñoz, Javier ; Cortés, Juan Carlos G ; Sipiczki, M. ; Ramos, Mariona ; Clemente-Ramos, José Angel ; Moreno, M. Belén ; Martins, Ivone M. ; Pérez, Pilar ; Ribas, Juan Carlos. / Extracellular cell wall β(1,3)glucan is required to couple septation to actomyosin ring contraction. In: Journal of Cell Biology. 2013 ; Vol. 203, No. 2. pp. 265-282.
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