Extensive flow cytometric characterization of plasmacytoid dendritic cell leukemia cells

Laszlo Gopcsa, Aniko Banyai, Katalin Jakab, Luca Kormos, Julia Tamaska, Andras Matolcsy, Peter Gogolak, Eva Rajnavolgyi, Katalin Paloczi

Research output: Article

32 Citations (Scopus)

Abstract

Objectives: Accumulating evidence suggests that non-T, non-B cell CD4+ CD56+ neoplasms with lymphoblastic morphology include clinically and immunophenotypically diverse entities. Although their cells of origin or classification are still controversial several entities clearly represent a distinct type of neoplasms that are clinically aggressive. Methods: In this work we present the immunophenotypic and genotypic features of bone marrow (BM), peripheral blood (PB), lymph node and skin lymphocytes from a patient diagnosed as plasmacytoid dendritic cell leukemia involving the skin, BM, PB, lymph nodes, liver and spleen. For determination of immunophenotypic characteristics of malignant plasmacytoid dendritic cells 73 monoclonal antibodies detecting lineage markers, chemokine receptors, cytokine receptors, activation, and co-stimulatory molecules were used. Results and conclusion: The malignant cells proved to express CD4+, CD56+ lineage negative leukemia phenotype characteristically positive for CD36, CD38, CD40, CD45, CD45RA, CD68, CD123, CD184, HLA-DR, BDCA2, and granzyme-B corresponding to the preplasmacytoid dendritic cell developmental stage. The presence of CD11a/CD18, CD84, CD91, CD95, αvβ5, CDw197, and the absence of CD52 and CD133 in this case can be regarded as additional features of malignant cells. Completing the immunophenotypes with multidrug resistance function can provide additional information for characterizing pDC leukemia.

Original languageEnglish
Pages (from-to)346-351
Number of pages6
JournalEuropean journal of haematology
Volume75
Issue number4
DOIs
Publication statusPublished - okt. 1 2005

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ASJC Scopus subject areas

  • Hematology

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