Expression pattern of Filamin-240 in Drosophila blood cells

Florentina Rus, Éva Kurucz, Róbert Márkus, Sergey A. Sinenko, Barbara Laurinyecz, Csilla Pataki, János Gausz, Zoltán Hegedus, Andor Udvardy, Dan Hultmark, István Andó

Research output: Article

13 Citations (Scopus)

Abstract

The expression pattern of Filamin-240 was studied in subsets of Drosophila blood cells by means of immunofluorescent staining and Western blot analysis with use of an antibody specific to a "filamin-folding domain", a consensus motif profile generated from the 20 existing filamin repeats. Expression of Filamin-240 is restricted to lamellocytes - a special blood cell type of the cellular immune response - and is involved in the regulation of lamellocyte development. In the cher1 homozygous larvae, which lack Filamin-240 protein, a vigorous lamellocyte differentiation occurs which is further enhanced upon in vivo immune challenge by a parasitic wasp, Leptopilina boulardi. By introducing a full-length transgene encoding the Drosophila Filamin-240 protein into the cher1 Filamin-deficient homozygous mutant, the mutant blood cell phenotype was rescued. These data demonstrate that the expression of Filamin-240 is strictly lamellocyte specific in Drosophila blood cells and that the protein is a suppressor of lamellocyte development.

Original languageEnglish
Pages (from-to)928-934
Number of pages7
JournalGene Expression Patterns
Volume6
Issue number8
DOIs
Publication statusPublished - okt. 2 2006

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ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Developmental Biology

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