Expression of the eRF1 translation termination factor is controlled by an autoregulatory circuit involving readthrough and nonsense-mediated decay in plants

Tünde Nyikó, Andor Auber, Levente Szabadkai, Anna Benkovics, Mariann Auth, Zsuzsanna Mérai, Z. Kerényi, Andrea Dinnyés, Ferenc Nagy, D. Silhavy

Research output: Article

8 Citations (Scopus)

Abstract

When a ribosome reaches a stop codon, the eukaryotic Release Factor 1 (eRF1) binds to the A site of the ribosome and terminates translation. In yeasts and plants, both over-and underexpression of eRF1 lead to altered phenotype indicating that eRF1 expression should be strictly controlled. However, regulation of eRF1 level is still poorly understood. Here we show that expression of plant eRF1 is controlled by a complex negative autoregulatory circuit, which is based on the unique features of the 3′.,untranslated region (3′.,UTR) of the eRF1-1 transcript. The stop codon of the eRF1-1 mRNA is in a translational readthrough promoting context, while its 3′.,UTR induces nonsense-mediated decay (NMD), a translation termination coupled mRNA degradation mechanism. We demonstrate that readthrough partially protects the eRF1-1 mRNA from its 3′.,UTR induced NMD, and that elevated eRF1 levels inhibit readthrough and stimulate NMD. Thus, high eRF1 level leads to reduced eRF1-1 expression, as weakened readthrough fails to protect the eRF1-1 mRNA from the more intense NMD. This eRF1 autoregulatory circuit might serve to finely balance general translation termination efficiency.

Original languageEnglish
Pages (from-to)4174-4188
Number of pages15
JournalNucleic Acids Research
Volume45
Issue number7
DOIs
Publication statusPublished - ápr. 20 2017

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Untranslated Regions
Terminator Codon
Ribosomes
Messenger RNA
RNA Stability
3' Untranslated Regions
Yeasts
Phenotype

ASJC Scopus subject areas

  • Genetics

Cite this

Expression of the eRF1 translation termination factor is controlled by an autoregulatory circuit involving readthrough and nonsense-mediated decay in plants. / Nyikó, Tünde; Auber, Andor; Szabadkai, Levente; Benkovics, Anna; Auth, Mariann; Mérai, Zsuzsanna; Kerényi, Z.; Dinnyés, Andrea; Nagy, Ferenc; Silhavy, D.

In: Nucleic Acids Research, Vol. 45, No. 7, 20.04.2017, p. 4174-4188.

Research output: Article

Nyikó, Tünde ; Auber, Andor ; Szabadkai, Levente ; Benkovics, Anna ; Auth, Mariann ; Mérai, Zsuzsanna ; Kerényi, Z. ; Dinnyés, Andrea ; Nagy, Ferenc ; Silhavy, D. / Expression of the eRF1 translation termination factor is controlled by an autoregulatory circuit involving readthrough and nonsense-mediated decay in plants. In: Nucleic Acids Research. 2017 ; Vol. 45, No. 7. pp. 4174-4188.
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