Experimental system for studying long-term drought stress adaptation of wheat cultivars

Maria Secenji, Ágnes Lendvai, Zsuzsa Hajósné, D. Dudits, J. Györgyey

Research output: Article

6 Citations (Scopus)

Abstract

Water limitation is a well-known problem for plants. Lack of water affects their biomass, their yield that is the most conspicuous in case of crops causing severe uncertainty of agricultural productivity. Under drought stress, plants generally display many physiological responses such as stomata closure, decreased/stopped photosynthetic activity, increased root/shoot ratio, reduced growth of vegetative parts. Many of the physiological changes are caused by underlying transcriptional alterations of high number of genes in many cases. One of the most studied phenomenon is the accumulation of proline as an osmoprotectant. Proline biosynthesis is increased by water deficit due to increased expression of the key enzyme, namely Δ 1-pyrroline-5-carboxylate synthetase (P5CS). In our experiments, we applied P5CS as a positive control to evaluate our new experimental system, which will allow to follow transcriptional changes in shoots, as well as in roots during drought adaptation. Our alternative approach allows greenhouse or growth chamber experiments that are more similar to natural conditions than the widely used experimental systems based on osmotic agents such as polyethylene glycol (PEG).

Original languageEnglish
Pages (from-to)51-52
Number of pages2
JournalActa Biologica Szegediensis
Volume49
Issue number1-2
Publication statusPublished - 2005

Fingerprint

Drought
Droughts
Triticum
water stress
Ligases
ligases
Proline
wheat
Water
proline
cultivars
osmotolerance
water
Biosynthesis
Greenhouses
root shoot ratio
Growth
polyethylene glycol
growth chambers
stomata

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology
  • Neuroscience(all)
  • Applied Microbiology and Biotechnology

Cite this

Experimental system for studying long-term drought stress adaptation of wheat cultivars. / Secenji, Maria; Lendvai, Ágnes; Hajósné, Zsuzsa; Dudits, D.; Györgyey, J.

In: Acta Biologica Szegediensis, Vol. 49, No. 1-2, 2005, p. 51-52.

Research output: Article

Secenji, Maria ; Lendvai, Ágnes ; Hajósné, Zsuzsa ; Dudits, D. ; Györgyey, J. / Experimental system for studying long-term drought stress adaptation of wheat cultivars. In: Acta Biologica Szegediensis. 2005 ; Vol. 49, No. 1-2. pp. 51-52.
@article{0969fcbbbbe7423cb027308058e9bb43,
title = "Experimental system for studying long-term drought stress adaptation of wheat cultivars",
abstract = "Water limitation is a well-known problem for plants. Lack of water affects their biomass, their yield that is the most conspicuous in case of crops causing severe uncertainty of agricultural productivity. Under drought stress, plants generally display many physiological responses such as stomata closure, decreased/stopped photosynthetic activity, increased root/shoot ratio, reduced growth of vegetative parts. Many of the physiological changes are caused by underlying transcriptional alterations of high number of genes in many cases. One of the most studied phenomenon is the accumulation of proline as an osmoprotectant. Proline biosynthesis is increased by water deficit due to increased expression of the key enzyme, namely Δ 1-pyrroline-5-carboxylate synthetase (P5CS). In our experiments, we applied P5CS as a positive control to evaluate our new experimental system, which will allow to follow transcriptional changes in shoots, as well as in roots during drought adaptation. Our alternative approach allows greenhouse or growth chamber experiments that are more similar to natural conditions than the widely used experimental systems based on osmotic agents such as polyethylene glycol (PEG).",
keywords = "Drought stress, Long-term adaptation, P5CS, Wheat",
author = "Maria Secenji and {\'A}gnes Lendvai and Zsuzsa Haj{\'o}sn{\'e} and D. Dudits and J. Gy{\"o}rgyey",
year = "2005",
language = "English",
volume = "49",
pages = "51--52",
journal = "Acta Biologica Szegediensis",
issn = "1588-385X",
publisher = "University of Szeged",
number = "1-2",

}

TY - JOUR

T1 - Experimental system for studying long-term drought stress adaptation of wheat cultivars

AU - Secenji, Maria

AU - Lendvai, Ágnes

AU - Hajósné, Zsuzsa

AU - Dudits, D.

AU - Györgyey, J.

PY - 2005

Y1 - 2005

N2 - Water limitation is a well-known problem for plants. Lack of water affects their biomass, their yield that is the most conspicuous in case of crops causing severe uncertainty of agricultural productivity. Under drought stress, plants generally display many physiological responses such as stomata closure, decreased/stopped photosynthetic activity, increased root/shoot ratio, reduced growth of vegetative parts. Many of the physiological changes are caused by underlying transcriptional alterations of high number of genes in many cases. One of the most studied phenomenon is the accumulation of proline as an osmoprotectant. Proline biosynthesis is increased by water deficit due to increased expression of the key enzyme, namely Δ 1-pyrroline-5-carboxylate synthetase (P5CS). In our experiments, we applied P5CS as a positive control to evaluate our new experimental system, which will allow to follow transcriptional changes in shoots, as well as in roots during drought adaptation. Our alternative approach allows greenhouse or growth chamber experiments that are more similar to natural conditions than the widely used experimental systems based on osmotic agents such as polyethylene glycol (PEG).

AB - Water limitation is a well-known problem for plants. Lack of water affects their biomass, their yield that is the most conspicuous in case of crops causing severe uncertainty of agricultural productivity. Under drought stress, plants generally display many physiological responses such as stomata closure, decreased/stopped photosynthetic activity, increased root/shoot ratio, reduced growth of vegetative parts. Many of the physiological changes are caused by underlying transcriptional alterations of high number of genes in many cases. One of the most studied phenomenon is the accumulation of proline as an osmoprotectant. Proline biosynthesis is increased by water deficit due to increased expression of the key enzyme, namely Δ 1-pyrroline-5-carboxylate synthetase (P5CS). In our experiments, we applied P5CS as a positive control to evaluate our new experimental system, which will allow to follow transcriptional changes in shoots, as well as in roots during drought adaptation. Our alternative approach allows greenhouse or growth chamber experiments that are more similar to natural conditions than the widely used experimental systems based on osmotic agents such as polyethylene glycol (PEG).

KW - Drought stress

KW - Long-term adaptation

KW - P5CS

KW - Wheat

UR - http://www.scopus.com/inward/record.url?scp=33644555237&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33644555237&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:33644555237

VL - 49

SP - 51

EP - 52

JO - Acta Biologica Szegediensis

JF - Acta Biologica Szegediensis

SN - 1588-385X

IS - 1-2

ER -