Experimental evidence for the influence of cumulus-oocyte-complexes on sperm release from the porcine oviductal sperm reservoir.

Klaus Peter Brüssow, Helmut Torner, J. Rátky, Noboru Manabe, Armin Tuchscherer

Research output: Article

13 Citations (Scopus)

Abstract

In the pig, a temporal relationship is suggested between sperm release from the sperm reservoir (SR) and ovulation, but the mechanism(s) is still under discussion. In two experiments, the influence of transferred ova on the release of SR-spermatozoa at ovulation and the effect of supplementation with non-sulfated glycosaminoglycan hyaluronan (HA) on embryo development and the number of accessory spermatozoa, respectively, were examined. PMSG/hCG primed ovectomized gilts that had previously received endoscopic low-dose insemination into the cranial uterine horn were used as an experimental model. After salpingectomy, tubal segments (ampulla, cranial, and caudal isthmus) were flushed and sperm numbers or respective accessory spermatozoa were counted. In Experiment 1, the distribution of the sperm population was altered in the presence of cumulus-oocyte-complexes (COCs). A higher proportion of spermatozoa was found after transfer of COCs into one oviduct in the ampulla and cranial isthmus segments compared with the controls (17.5 vs. 4.9%, p

Original languageEnglish
Pages (from-to)249-257
Number of pages9
JournalJournal of Reproduction and Development
Volume52
Issue number2
DOIs
Publication statusPublished - ápr. 2006

Fingerprint

spermiation
Oocytes
Spermatozoa
oocytes
Swine
spermatozoa
swine
Ovulation
ovulation
hyaluronic acid
Salpingectomy
glycosaminoglycans
Sperm Count
Oviducts
oviducts
ova
Insemination
gilts
insemination
Hyaluronic Acid

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Reproductive Medicine

Cite this

Experimental evidence for the influence of cumulus-oocyte-complexes on sperm release from the porcine oviductal sperm reservoir. / Brüssow, Klaus Peter; Torner, Helmut; Rátky, J.; Manabe, Noboru; Tuchscherer, Armin.

In: Journal of Reproduction and Development, Vol. 52, No. 2, 04.2006, p. 249-257.

Research output: Article

Brüssow, Klaus Peter ; Torner, Helmut ; Rátky, J. ; Manabe, Noboru ; Tuchscherer, Armin. / Experimental evidence for the influence of cumulus-oocyte-complexes on sperm release from the porcine oviductal sperm reservoir. In: Journal of Reproduction and Development. 2006 ; Vol. 52, No. 2. pp. 249-257.
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