The authors have given a report on the activities of the first Hungarian PCR laboratory in the veterinary diagnostic. Model experiments were carried out in adenoviruses. It was found that after the application of a so-called wide-spectrum primer, serving for the demonstration of both human and animal adenoviruses, typifying of viruses by endonuclease enzymes was possible. A simple, effective sample processing method was elaborated for the demonstration of Rubarth's adenovirus of dogs. In case of other, economically important pathogens, a similar method was elaborated for the diagnosis of avian mycoplasmoses. PCR technique was usefully applied for solving the uncertainties and contradictions in the diagnosis of Derzsy's disease. Of the RNA viruses, PCR technique was used for the demonstration and identification of pestiviruses (classical swine fever and bovine virus diarrhoea viruses) after reverse transcription of the virus genome (RT-PCR). Classical swine fever virus was successfully demonstrated by RT-PCR from leukocytes and other samples unfit for other investigations even within one day. Bovine virus diarrhoea virus was demonstraded by similar procedure, however by primers specific for other sites of the virus genome. They have been working on the development of such PCR systems using the genome structural knowledge cumulating nowadays that besides the genotypifying it can make also possible the determination of recombination types in the future. It has been pointed out that PCR serving diagnostic purposes, because of the false positive and negative reactions, should be applied carefully. The technique is a valuable complementary method of the traditional laboratory techniques, however they cannot substitute the isolation of virus which is essential because of ensuring possibilities for further morphological analyses and revealing functional relationships.
|Translated title of the contribution||Experiences of using the PCR at the veterinary institute of debrecen|
|Number of pages||3|
|Journal||Magyar Allatorvosok Lapja|
|Publication status||Published - dec. 1 1997|
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