Evidence for pituitary-brain transport of a behaviorally potent acth analog

Eva Mezey, M. Palkóvits, E. R. de Kloet, J. Verhoef, D. de Wied

Research output: Article

94 Citations (Scopus)

Abstract

Following intrapituitary injection of 3H-ACTH 4-9 analog, the radioactivity of various brain regions was determined in intact rats and in rats with the pituitary stalk cut one or eight days previously. The regional distribution of radioactivity in the brain was also investigated after intravenous and intrasellar administration. Intrasellar and intrapituitary administration resulted in significantly higher radioactivity levels in the brain than did intravenous injection of an equimolar dose of labeled peptide. Intrapituitary injection resulted in an uptake with clear regional differences and which was highest in the hypothalamus. Twenty four hours after stalk section the uptake of radioactivity in the hypothalamus, but not in other brain regions was markedly depressed. Hypothalamic uptake, however, was restored at eight days after stalk section. The results suggest a significant flow of radioactivity from the pituitary to the brain, particularly to the hypothalamus. Transport to the hypothalamus is presumably partly vascular via the stalk. Transport to other brain areas may occur via the cerebrospinal fluid, but a neural route cannot be excluded.

Original languageEnglish
Pages (from-to)831-838
Number of pages8
JournalLife Sciences
Volume22
Issue number10
DOIs
Publication statusPublished - 1978

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Radioactivity
Brain
Hypothalamus
Rats
Cerebrospinal fluid
Injections
Pituitary Gland
Intravenous Injections
Intravenous Administration
Blood Vessels
Cerebrospinal Fluid
Peptides

ASJC Scopus subject areas

  • Pharmacology

Cite this

Evidence for pituitary-brain transport of a behaviorally potent acth analog. / Mezey, Eva; Palkóvits, M.; de Kloet, E. R.; Verhoef, J.; de Wied, D.

In: Life Sciences, Vol. 22, No. 10, 1978, p. 831-838.

Research output: Article

Mezey, Eva ; Palkóvits, M. ; de Kloet, E. R. ; Verhoef, J. ; de Wied, D. / Evidence for pituitary-brain transport of a behaviorally potent acth analog. In: Life Sciences. 1978 ; Vol. 22, No. 10. pp. 831-838.
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AU - de Wied, D.

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