Evaluation of sperm tail membrane integrity by light microscopy

S. Nagy, G. Házas, Á Bali Papp, J. Iváncsics, F. Szász, F. Szász, A. Kovács, R. H. Foote

Research output: Article

25 Citations (Scopus)

Abstract

During routine evaluation of trypan blue-Giemsa stained semen smears, sperm cells can be found with unstained heads and with stained tails. It was hypothesized that these cells were immotile and should not be considered as live. Sperm motility was determined in isoosmotic, and presumably isotonic trypan blue-stained wet preparations. Bull, ram and boar semen smears were stained with hypoosmotic trypan blue-Giemsa to compare the relationship between the percentage of stained sperm tails and the percentage of sperm tails remaining straight under hypoosmotic conditions. Actively moving spermatozoa with unstained heads, but with stained tails were never observed in wet preparations. The correlation coefficient found between the percentage of sperm with stained tails and the percentage with straight tails was 0.81, 0.94 and 0.85 for bull, ram and boar spermatozoa, respectively. Results of this study show that sperm cells with an intact head membrane, but a stained and presumably membrane-damaged tail are not motile. Therefore these cells should be included in the dead category rather than alive in the usual live- dead studies with vital stains.

Original languageEnglish
Pages (from-to)1153-1159
Number of pages7
JournalTheriogenology
Volume52
Issue number7
DOIs
Publication statusPublished - nov. 1999

Fingerprint

Sperm Tail
Spermatozoa
light microscopy
Microscopy
tail
spermatozoa
Trypan Blue
Light
Membranes
Semen
Head
boars
rams
semen
bulls
Sperm Motility
cells
Coloring Agents
sperm motility

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Nagy, S., Házas, G., Bali Papp, Á., Iváncsics, J., Szász, F., Szász, F., ... Foote, R. H. (1999). Evaluation of sperm tail membrane integrity by light microscopy. Theriogenology, 52(7), 1153-1159. https://doi.org/10.1016/S0093-691X(99)00207-1

Evaluation of sperm tail membrane integrity by light microscopy. / Nagy, S.; Házas, G.; Bali Papp, Á; Iváncsics, J.; Szász, F.; Szász, F.; Kovács, A.; Foote, R. H.

In: Theriogenology, Vol. 52, No. 7, 11.1999, p. 1153-1159.

Research output: Article

Nagy, S, Házas, G, Bali Papp, Á, Iváncsics, J, Szász, F, Szász, F, Kovács, A & Foote, RH 1999, 'Evaluation of sperm tail membrane integrity by light microscopy', Theriogenology, vol. 52, no. 7, pp. 1153-1159. https://doi.org/10.1016/S0093-691X(99)00207-1
Nagy S, Házas G, Bali Papp Á, Iváncsics J, Szász F, Szász F et al. Evaluation of sperm tail membrane integrity by light microscopy. Theriogenology. 1999 nov.;52(7):1153-1159. https://doi.org/10.1016/S0093-691X(99)00207-1
Nagy, S. ; Házas, G. ; Bali Papp, Á ; Iváncsics, J. ; Szász, F. ; Szász, F. ; Kovács, A. ; Foote, R. H. / Evaluation of sperm tail membrane integrity by light microscopy. In: Theriogenology. 1999 ; Vol. 52, No. 7. pp. 1153-1159.
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