Evaluation and modulation of selectivity in reversed-phase high-performance liquid chromatography

Ákos Sándi, László Szepesy

Research output: Article

46 Citations (Scopus)


Retention factors for 31 solutes of widely different types were determined on five columns of different characteristics using acetonitrile-water and methanol-water as the mobile phases, in a composition range of 20-70% (v/v) of organic modifiers. Calculating selectivity factors, α, for various types of solute pairs, different types of selectivites were determined and evaluated as hydrophobic or methylene selectivity, chemical or polar selectivity and relative retention of different types of polar solutes. It was demonstrated that chromatographic selectivity is a very complex phenomenon depending on the characteristics of the stationary phase, the type of the organic modifier, the composition of the mobile phase and also on the type and structural characteristics of the compounds to be separated. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)113-131
Number of pages19
JournalJournal of Chromatography A
Issue number1-2
Publication statusPublished - jún. 11 1999

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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