Estimating genome conservation between crop and model legume species

Hong Kyu Choi, Jeong Hwan Mun, Dong Jin Kim, Hongyan Zhu, Jong Min Baek, Joanne Mudge, Bruce Roe, Noel Ellis, Jeff Doyle, G. Kiss, Nevin D. Young, Douglas R. Cook

Research output: Article

375 Citations (Scopus)

Abstract

Legumes are simultaneously one of the largest families of crop plants and a cornerstone in the biological nitrogen cycle. We combined molecular and phylogenetic analyses to evaluate genome conservation both within and between the two major clades of crop legumes. Genetic mapping of orthologous genes identifies broad conservation of genome macrostructure, especially within the galegoid legumes, while also highlighting inferred chromosomal rearrangements that may underlie the variation in chromosome number between these species. As a complement to comparative genetic mapping, we compared sequenced regions of the model legume Medicago truncatula with those of the diploid Lotus japonicus and the polyploid Glycine max. High conservation was observed between the genomes of M. truncatula and L. japonicus, whereas lower levels of conservation were evident between M. truncatula and G. max. In all cases, conserved genome microstructure was punctuated by significant structural divergence, including frequent insertion/deletion of individual genes or groups of genes and lineage-specific expansion/contraction of gene families. These results suggest that comparative mapping may have considerable utility for basic and applied research in the legumes, although its predictive value is likely to be tempered by phylogenetic distance and genome duplication.

Original languageEnglish
Pages (from-to)15289-15294
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume101
Issue number43
DOIs
Publication statusPublished - okt. 26 2004

Fingerprint

Fabaceae
Medicago truncatula
Genome
Soybeans
Nitrogen Cycle
Polyploidy
Chromosome Mapping
Gene Deletion
Diploidy
Genes
Chromosomes
Research
Loteae

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Estimating genome conservation between crop and model legume species. / Choi, Hong Kyu; Mun, Jeong Hwan; Kim, Dong Jin; Zhu, Hongyan; Baek, Jong Min; Mudge, Joanne; Roe, Bruce; Ellis, Noel; Doyle, Jeff; Kiss, G.; Young, Nevin D.; Cook, Douglas R.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, No. 43, 26.10.2004, p. 15289-15294.

Research output: Article

Choi, HK, Mun, JH, Kim, DJ, Zhu, H, Baek, JM, Mudge, J, Roe, B, Ellis, N, Doyle, J, Kiss, G, Young, ND & Cook, DR 2004, 'Estimating genome conservation between crop and model legume species', Proceedings of the National Academy of Sciences of the United States of America, vol. 101, no. 43, pp. 15289-15294. https://doi.org/10.1073/pnas.0402251101
Choi, Hong Kyu ; Mun, Jeong Hwan ; Kim, Dong Jin ; Zhu, Hongyan ; Baek, Jong Min ; Mudge, Joanne ; Roe, Bruce ; Ellis, Noel ; Doyle, Jeff ; Kiss, G. ; Young, Nevin D. ; Cook, Douglas R. / Estimating genome conservation between crop and model legume species. In: Proceedings of the National Academy of Sciences of the United States of America. 2004 ; Vol. 101, No. 43. pp. 15289-15294.
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