Enzyme-linked immunosorbent assay in the serodiagnosis of syphilis.

M. Marschalkó, I. Barna-Vetró, I. Horváth

Research output: Article

Abstract

Enzyme-linked immunosorbent assay using ultrasonic lysate of Treponema pallidum and Treponema reiteri as antigens was used for the detection of antisyphilitic antibodies in various stages of syphilis. The conjugate was goat antiserum to human IgG labelled with horseradish peroxidase. A comparison with results of the T. pallidum immobilization test, Rapid Plasma Reagin test, Kolmer complement fixation reaction using cardiolipin and Reiter protein antigens showed that ELISA was more sensitive but less specific with T. pallidum antigen, whereas less sensitive but more specific with T. reiteri antigen. Absorption of group specific treponemal antibodies was needed to make the method reliable.

Original languageEnglish
Pages (from-to)221-226
Number of pages6
JournalActa Microbiologica Academiae Scientiarum Hungaricae
Volume29
Issue number4
Publication statusPublished - 1982

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Syphilis Serodiagnosis
Viral Tumor Antigens
Antitreponemal Agents
Treponema Immobilization Test
Enzyme-Linked Immunosorbent Assay
Treponema
Reagins
Treponema pallidum
Antigens
Complement Fixation Tests
Cardiolipins
Antibodies
Horseradish Peroxidase
Syphilis
Goats
Ultrasonics
Immune Sera
Immunoglobulin G
Proteins

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Enzyme-linked immunosorbent assay in the serodiagnosis of syphilis. / Marschalkó, M.; Barna-Vetró, I.; Horváth, I.

In: Acta Microbiologica Academiae Scientiarum Hungaricae, Vol. 29, No. 4, 1982, p. 221-226.

Research output: Article

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