Enhancement of mustard oil yield by cellulolytic pretreatment

M. Szakács-Dobozi, A. Halász, E. Kozma-Kovács, G. Szakács

Research output: Article

15 Citations (Scopus)

Abstract

One part by weight of finely powdered expeller cake from brown mustard seeds (Brassica juncea) was mixed well with 2.5 parts by weight of water and, after addition of 0.2% CelluclastTM (NOVO Industry A/S, Bagsvaerd), the mixture was allowed to stand at 20°C, 35°C or 50°C for 2 h. The enzymatic breakdown was followed using a reductometric method. The mixture was then mixed well with one part by volume of water, the temperature was rapidly raised to 100°C, and the volatile oil was recovered by steam distillation. The yield of the resulting oil from ground samples was approximately 50% higher than that obtained without cellulolytic pretreatment at 35°C. Grinding of the expeller cake enhanced enzymatic digestion. The allyl isothiocyanate content of the oil was estimated by titrimetric and gas-chromatographic methods.

Original languageEnglish
Pages (from-to)39-43
Number of pages5
JournalApplied Microbiology and Biotechnology
Volume29
Issue number1
DOIs
Publication statusPublished - aug. 1988

Fingerprint

Mustard Plant
Oils
Weights and Measures
Distillation
Water
Steam
Volatile Oils
Seed
Digestion
Seeds
Industry
Gases
Temperature
mustard oil
allyl isothiocyanate

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology
  • Bioengineering
  • Biotechnology

Cite this

Enhancement of mustard oil yield by cellulolytic pretreatment. / Szakács-Dobozi, M.; Halász, A.; Kozma-Kovács, E.; Szakács, G.

In: Applied Microbiology and Biotechnology, Vol. 29, No. 1, 08.1988, p. 39-43.

Research output: Article

Szakács-Dobozi, M. ; Halász, A. ; Kozma-Kovács, E. ; Szakács, G. / Enhancement of mustard oil yield by cellulolytic pretreatment. In: Applied Microbiology and Biotechnology. 1988 ; Vol. 29, No. 1. pp. 39-43.
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