The effect of electric field strength gradients on the separation of DNA restriction fragments was Investigated. As reported In our earlier work, the mobility of different size double-stranded DNA molecules Is a function of the applied electric field which suggests that the use of a nonunlform (time varying) electric field may Increase the resolving power. We demonstrate that In capillary gel electrophoresis enhanced separation of DNA restriction fragments up to 1353 base pairs (bp) In size can be achieved by employing the field strength gradient method. The shape of the gradient can be continuous or stepwise over time. Both methods can be used to increase separation efficiency and resolution in capillary gel electrophoresis of double-stranded DNA molecules.
ASJC Scopus subject areas
- Analytical Chemistry