Endomorphin-2, an endogenous tetrapeptide, protects against Aβ1-42 in vitro and in vivo

Viktor Szegedi, Gábor Juhá, Éva Rózsa, Gabriella Juhász-Vedres, Zsolt Datki, Lívia Fülöp, Zsolt Bozsó, Andrea Lakatos, Ilona Laczkó, Tamás Farkas, Zsolt Kis, Géza Tóth, Katalin Soós, Márta Zarándi, Dénes Budai, József Toldi, Botond Penke

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21 Citations (Scopus)

Abstract

The underlying cause of Alzheimer's disease (AD) is thought to be the β-amyloid aggregates formed mainly by Aβ1-42 peptide. Protective pentapeptides [e.g., Leu-Pro-Phe-Phe-Asp (LPFFD)] have been shown to prevent neuronal toxicity of Aβ1-42 by arresting and reversing fibril formation. Here we report that an endogenous tetrapeptide, endomorphin-2 (End-2, amino acid sequence: YPFF), defends against Aβ1-42 induced neuromodulatory effects at the cellular level. Although End-2 does not interfere with the kinetics of Aβ fibrillogenesis according to transmission electron microscopic studies and quasielastic light scattering measurements, it binds to Aβ1-42 during aggregation, as revealed by tritium-labeled End-2 binding assay and circular dichroism measurements. The tetrapeptide attenuates the inhibitory effect on cellular redox activity of Aβ1-42 in a dose-dependent manner, as measured by 3-(4,5-dimethylthiazolyl-2)-2,-5-diphenyltetrazolium bromide (MTT) assay. In vitro and in vivo electrophysiological experiments show that End-2 also protects against the field excitatory postsynaptic potential attenuating and the NMDA-evoked response-enhancing effect of Aβ1-42. Studies using [D-Ala (2), N-Me-Phe (4), Gly (5)-ol]-enkephalin (DAMGO), a μ-opioid receptor agonist, show that the protective effects of the tetrapeptide are not μ-receptor modulated. The endogenous tetrapeptide End-2 may serve as a lead compound for the drug development in the treatment of AD.

Original languageEnglish
Pages (from-to)E324-E333
JournalFASEB Journal
Volume20
Issue number8
DOIs
Publication statusPublished - jún. 1 2006

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ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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