EMMPRIN overexpression in SVZ neural progenitor cells increases their migration towards ischemic cortex

Michiko Kanemitsu, Oleg Tsupykov, Gaël Potter, Michael Boitard, Patrick Salmon, Eloisa Zgraggen, Eduardo Gascon, Galina Skibo, Alexandre G. Dayer, J. Kiss

Research output: Article

4 Citations (Scopus)

Abstract

Stimulation of endogenous neurogenesis and recruitment of neural progenitors from the subventricular zone (SVZ) neurogenic site may represent a useful strategy to improve regeneration in the ischemic cortex. Here, we tested whether transgenic overexpression of extracellular matrix metalloproteinase inducer (EMMPRIN), the regulator of matrix metalloproteinases (MMPs) expression, in endogenous neural progenitor cells (NPCs) in the subventricular zone (SVZ) could increase migration towards ischemic injury. For this purpose, we applied a lentivector-mediated gene transfer system. We found that EMMPRIN-transduced progenitors exhibited enhanced MMP-2 activity in vitro and showed improved motility in 3D collagen gel as well as in cortical slices. Using a rat model of neonatal ischemia, we showed that EMMPRIN overexpressing SVZ cells invade the injured cortical tissue more efficiently than controls. Our results suggest that EMMPRIN overexpression could be suitable approach to improve capacities of endogenous or transplanted progenitors to invade the injured cortex.

Original languageEnglish
Pages (from-to)14-24
Number of pages11
JournalExperimental Neurology
Volume297
DOIs
Publication statusPublished - nov. 1 2017

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience

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    Kanemitsu, M., Tsupykov, O., Potter, G., Boitard, M., Salmon, P., Zgraggen, E., Gascon, E., Skibo, G., Dayer, A. G., & Kiss, J. (2017). EMMPRIN overexpression in SVZ neural progenitor cells increases their migration towards ischemic cortex. Experimental Neurology, 297, 14-24. https://doi.org/10.1016/j.expneurol.2017.07.009