Electrospun polylactic acid and polyvinyl alcohol fibers as efficient and stable nanomaterials for immobilization of lipases

Péter Lajos Sóti, Diana Weiser, Tamás Vigh, Zsombor Kristóf Nagy, L. Poppe, G. Marosi

Research output: Article

19 Citations (Scopus)

Abstract

Electrospinning was applied to create easy-to-handle and high-surface-area membranes from continuous nanofibers of polyvinyl alcohol (PVA) or polylactic acid (PLA). Lipase PS from Burkholderia cepacia and Lipase B from Candida antarctica (CaLB) could be immobilized effectively by adsorption onto the fibrous material as well as by entrapment within the electrospun nanofibers. The biocatalytic performance of the resulting membrane biocatalysts was evaluated in the kinetic resolution of racemic 1-phenylethanol (rac-1) and 1-phenylethyl acetate (rac-2). Fine dispersion of the enzymes in the polymer matrix and large surface area of the nanofibers resulted in an enormous increase in the activity of the membrane biocatalyst compared to the non-immobilized crude powder forms of the lipases. PLA as fiber-forming polymer for lipase immobilization performed better than PVA in all aspects. Recycling studies with the various forms of electrospun membrane biocatalysts in ten cycles of the acylation and hydrolysis reactions indicated excellent stability of this forms of immobilized lipases. PLA-entrapped lipases could preserve lipase activity and enantiomer selectivity much better than the PVA-entrapped forms. The electrospun membrane forms of CaLB showed high mechanical stability in the repeated acylations and hydrolyses than commercial forms of CaLB immobilized on polyacrylamide beads (Novozyme 435 and IMMCALB-T2-150).

Original languageEnglish
Pages (from-to)449-459
Number of pages11
JournalBioprocess and Biosystems Engineering
Volume39
Issue number3
DOIs
Publication statusPublished - márc. 1 2016

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Polyvinyl Alcohol
Nanostructures
Polyvinyl alcohols
Lipases
Lipase
Nanostructured materials
Immobilization
Nanofibers
Acids
Fibers
Membranes
Biocatalysts
Acylation
Enzymes
Hydrolysis
Polymers
Burkholderia cepacia
Enantiomers
Mechanical stability
Electrospinning

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

Cite this

Electrospun polylactic acid and polyvinyl alcohol fibers as efficient and stable nanomaterials for immobilization of lipases. / Sóti, Péter Lajos; Weiser, Diana; Vigh, Tamás; Nagy, Zsombor Kristóf; Poppe, L.; Marosi, G.

In: Bioprocess and Biosystems Engineering, Vol. 39, No. 3, 01.03.2016, p. 449-459.

Research output: Article

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AU - Sóti, Péter Lajos

AU - Weiser, Diana

AU - Vigh, Tamás

AU - Nagy, Zsombor Kristóf

AU - Poppe, L.

AU - Marosi, G.

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