Electrochemical and Photometric Detection of Plasmin by Specific Peptide Substrate

Gabriela Castillo, Kinga Pribransky, Gábor Mezo, László Kocsis, Antal Csámpai, Krisztina Németh, Zsófia Keresztes, Tibor Hianik

Research output: Article

9 Citations (Scopus)

Abstract

We report a comparative analysis of plasmin detection by means of electrochemical biosensor and photometric assays. The biosensor has been based on ferrocene-modified peptide (Fc-P) specific to plasmin. Fc-P substrate is enzymatically cleaved by plasmin and the short fragment of peptide contained Fc is released causing decrease of redox current measured by cyclic voltammetry. The biosensor was tested in the range 1-20nM of freshly prepared plasmin and it was highly selective (LOD=0.56±0.03nM) demonstrating lower reaction to other proteins such as thrombin and β-casein. The concentration of plasmin has been determined also by a photometric analysis based on UV-vis spectroscopy (LOD=3.68±0.04nM). The assay has been validated in milk samples.

Original languageEnglish
Pages (from-to)789-798
Number of pages10
JournalElectroanalysis
Volume27
Issue number3
DOIs
Publication statusPublished - márc. 1 2015

ASJC Scopus subject areas

  • Analytical Chemistry
  • Electrochemistry

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