Effects of PACAP and preconditioning against ischemia/reperfusion-induced cardiomyocyte apoptosis in vitro

Research output: Conference contribution

15 Citations (Scopus)

Abstract

The neuropeptide pituitary adenylate cyclase activating polypeptide (PACAP) and its receptors are widely expressed in the nervous system and various other tissues. PACAP exerts strong anti-apoptotic effects in neuronal cell lines and, according to recent data, also in non-neuronal cells. The peptide is present in the cardiovascular system and has various distinct effects. We have demonstrated earlier that PACAP has protective effects against in vitro ischemia/ reperfusion-induced apoptosis in cardiomyocytes. Preconditioning with brief intermittent periods of ischemia is known to provide protection against ischemic injury. The aim of the present study was to investigate whether PACAP could enhance the protective effect of preconditioning against in vitro ischemic injury. Cultured cardiomyocytes were exposed to brief preconditioning ischemia followed by 2 h ischemia and 4 h reperfusion. Both PACAP treatment and preconditioning alone significantly increased cell viability and decreased the ratio of cell death. Pretreatment with PACAP was found to further reduce the level of cleaved caspase-8 but it did not lead to additional survival rate when compared to cells treated with PACAP or preconditioning alone. These results show that although both PACAP and preconditioning have a protective effect against ischemia/reperfusion-induced cardiomyocyte apoptosis, their effects are not additive.

Original languageEnglish
Title of host publicationAnnals of the New York Academy of Sciences
Pages512-516
Number of pages5
Volume1163
DOIs
Publication statusPublished - ápr. 2009

Publication series

NameAnnals of the New York Academy of Sciences
Volume1163
ISSN (Print)00778923
ISSN (Electronic)17496632

Fingerprint

Pituitary Adenylate Cyclase-Activating Polypeptide
Cardiac Myocytes
Reperfusion
Ischemia
Apoptosis
Pituitary Adenylate Cyclase-Activating Polypeptide Receptors
Cells
Cardiovascular system
Caspase 8
Wounds and Injuries
Neurology
Cell death
Cardiovascular System
In Vitro Techniques
Neuropeptides
Nervous System
Cell Survival
Cell Death
Tissue
Cell Line

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Rőth, E., Wéber, G., Kiss, P., Horváth, G., Tóth, G., Gasz, B., ... Rácz, B. (2009). Effects of PACAP and preconditioning against ischemia/reperfusion-induced cardiomyocyte apoptosis in vitro. In Annals of the New York Academy of Sciences (Vol. 1163, pp. 512-516). (Annals of the New York Academy of Sciences; Vol. 1163). https://doi.org/10.1111/j.1749-6632.2008.03635.x

Effects of PACAP and preconditioning against ischemia/reperfusion-induced cardiomyocyte apoptosis in vitro. / Rőth, E.; Wéber, G.; Kiss, P.; Horváth, G.; Tóth, G.; Gasz, B.; Ferencz, A.; Gallyas, F.; Regløacdi, D.; Rácz, B.

Annals of the New York Academy of Sciences. Vol. 1163 2009. p. 512-516 (Annals of the New York Academy of Sciences; Vol. 1163).

Research output: Conference contribution

Rőth, E, Wéber, G, Kiss, P, Horváth, G, Tóth, G, Gasz, B, Ferencz, A, Gallyas, F, Regløacdi, D & Rácz, B 2009, Effects of PACAP and preconditioning against ischemia/reperfusion-induced cardiomyocyte apoptosis in vitro. in Annals of the New York Academy of Sciences. vol. 1163, Annals of the New York Academy of Sciences, vol. 1163, pp. 512-516. https://doi.org/10.1111/j.1749-6632.2008.03635.x
Rőth E, Wéber G, Kiss P, Horváth G, Tóth G, Gasz B et al. Effects of PACAP and preconditioning against ischemia/reperfusion-induced cardiomyocyte apoptosis in vitro. In Annals of the New York Academy of Sciences. Vol. 1163. 2009. p. 512-516. (Annals of the New York Academy of Sciences). https://doi.org/10.1111/j.1749-6632.2008.03635.x
Rőth, E. ; Wéber, G. ; Kiss, P. ; Horváth, G. ; Tóth, G. ; Gasz, B. ; Ferencz, A. ; Gallyas, F. ; Regløacdi, D. ; Rácz, B. / Effects of PACAP and preconditioning against ischemia/reperfusion-induced cardiomyocyte apoptosis in vitro. Annals of the New York Academy of Sciences. Vol. 1163 2009. pp. 512-516 (Annals of the New York Academy of Sciences).
@inproceedings{1b37338522074e5b8936e6ee90974647,
title = "Effects of PACAP and preconditioning against ischemia/reperfusion-induced cardiomyocyte apoptosis in vitro",
abstract = "The neuropeptide pituitary adenylate cyclase activating polypeptide (PACAP) and its receptors are widely expressed in the nervous system and various other tissues. PACAP exerts strong anti-apoptotic effects in neuronal cell lines and, according to recent data, also in non-neuronal cells. The peptide is present in the cardiovascular system and has various distinct effects. We have demonstrated earlier that PACAP has protective effects against in vitro ischemia/ reperfusion-induced apoptosis in cardiomyocytes. Preconditioning with brief intermittent periods of ischemia is known to provide protection against ischemic injury. The aim of the present study was to investigate whether PACAP could enhance the protective effect of preconditioning against in vitro ischemic injury. Cultured cardiomyocytes were exposed to brief preconditioning ischemia followed by 2 h ischemia and 4 h reperfusion. Both PACAP treatment and preconditioning alone significantly increased cell viability and decreased the ratio of cell death. Pretreatment with PACAP was found to further reduce the level of cleaved caspase-8 but it did not lead to additional survival rate when compared to cells treated with PACAP or preconditioning alone. These results show that although both PACAP and preconditioning have a protective effect against ischemia/reperfusion-induced cardiomyocyte apoptosis, their effects are not additive.",
keywords = "Cleaved caspase-8, Ischemic injury, MAPK, PACAP, Preconditioning",
author = "E. Rőth and G. W{\'e}ber and P. Kiss and G. Horv{\'a}th and G. T{\'o}th and B. Gasz and A. Ferencz and F. Gallyas and D. Regl{\o}acdi and B. R{\'a}cz",
year = "2009",
month = "4",
doi = "10.1111/j.1749-6632.2008.03635.x",
language = "English",
isbn = "9781573316712",
volume = "1163",
series = "Annals of the New York Academy of Sciences",
pages = "512--516",
booktitle = "Annals of the New York Academy of Sciences",

}

TY - GEN

T1 - Effects of PACAP and preconditioning against ischemia/reperfusion-induced cardiomyocyte apoptosis in vitro

AU - Rőth, E.

AU - Wéber, G.

AU - Kiss, P.

AU - Horváth, G.

AU - Tóth, G.

AU - Gasz, B.

AU - Ferencz, A.

AU - Gallyas, F.

AU - Regløacdi, D.

AU - Rácz, B.

PY - 2009/4

Y1 - 2009/4

N2 - The neuropeptide pituitary adenylate cyclase activating polypeptide (PACAP) and its receptors are widely expressed in the nervous system and various other tissues. PACAP exerts strong anti-apoptotic effects in neuronal cell lines and, according to recent data, also in non-neuronal cells. The peptide is present in the cardiovascular system and has various distinct effects. We have demonstrated earlier that PACAP has protective effects against in vitro ischemia/ reperfusion-induced apoptosis in cardiomyocytes. Preconditioning with brief intermittent periods of ischemia is known to provide protection against ischemic injury. The aim of the present study was to investigate whether PACAP could enhance the protective effect of preconditioning against in vitro ischemic injury. Cultured cardiomyocytes were exposed to brief preconditioning ischemia followed by 2 h ischemia and 4 h reperfusion. Both PACAP treatment and preconditioning alone significantly increased cell viability and decreased the ratio of cell death. Pretreatment with PACAP was found to further reduce the level of cleaved caspase-8 but it did not lead to additional survival rate when compared to cells treated with PACAP or preconditioning alone. These results show that although both PACAP and preconditioning have a protective effect against ischemia/reperfusion-induced cardiomyocyte apoptosis, their effects are not additive.

AB - The neuropeptide pituitary adenylate cyclase activating polypeptide (PACAP) and its receptors are widely expressed in the nervous system and various other tissues. PACAP exerts strong anti-apoptotic effects in neuronal cell lines and, according to recent data, also in non-neuronal cells. The peptide is present in the cardiovascular system and has various distinct effects. We have demonstrated earlier that PACAP has protective effects against in vitro ischemia/ reperfusion-induced apoptosis in cardiomyocytes. Preconditioning with brief intermittent periods of ischemia is known to provide protection against ischemic injury. The aim of the present study was to investigate whether PACAP could enhance the protective effect of preconditioning against in vitro ischemic injury. Cultured cardiomyocytes were exposed to brief preconditioning ischemia followed by 2 h ischemia and 4 h reperfusion. Both PACAP treatment and preconditioning alone significantly increased cell viability and decreased the ratio of cell death. Pretreatment with PACAP was found to further reduce the level of cleaved caspase-8 but it did not lead to additional survival rate when compared to cells treated with PACAP or preconditioning alone. These results show that although both PACAP and preconditioning have a protective effect against ischemia/reperfusion-induced cardiomyocyte apoptosis, their effects are not additive.

KW - Cleaved caspase-8

KW - Ischemic injury

KW - MAPK

KW - PACAP

KW - Preconditioning

UR - http://www.scopus.com/inward/record.url?scp=65449180377&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=65449180377&partnerID=8YFLogxK

U2 - 10.1111/j.1749-6632.2008.03635.x

DO - 10.1111/j.1749-6632.2008.03635.x

M3 - Conference contribution

C2 - 19456402

AN - SCOPUS:65449180377

SN - 9781573316712

VL - 1163

T3 - Annals of the New York Academy of Sciences

SP - 512

EP - 516

BT - Annals of the New York Academy of Sciences

ER -