Effects of imatinib and nilotinib on the whole transcriptome of cultured murine osteoblasts

Gyöngyi Kirschner, Bernadett Balla, P. Horváth, Andrea Kövesdi, Gergely Lakatos, I. Takács, Zsolt Nagy, Bálint Tóbiás, Kristóf Árvai, János Pál Kósa, Péter Lakatos

Research output: Article

1 Citation (Scopus)

Abstract

Numerous clinical observations have confirmed that breakpoint cluster region-abelson fusion oncoprotein tyrosine kinase inhibitors used in leukemia treatment alter bone physiology in a complex manner. The aim of the present study was to analyze the whole transcriptome of cultured murine osteoblasts and determine the changes following treatment with imatinib and nilotinib using Sequencing by Oligonucleotide Ligation and Detection next generation RNA sequencing. This study also aimed to identify candidate signaling pathways and network regulators by multivariate Ingenuity Pathway Analysis. Based on the right-tailed Fisher's exact test, significantly altered pathways including upstream regulators were defined for each drug. The correlation between these pathways and bone metabolism was also examined. The preliminary results suggest the two drugs have different mechanisms of action on osteoblasts, and imatinib was shown to have a greater effect on gene expression. Data also indicated the potential role of a number of genes and signaling cascades that may contribute to identifying novel targets for the treatment of metabolic bone diseases.

Original languageEnglish
Pages (from-to)2025-2037
Number of pages13
JournalMolecular Medicine Reports
Volume14
Issue number3
DOIs
Publication statusPublished - szept. 1 2016

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Oncology
  • Genetics
  • Cancer Research

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  • Cite this

    Kirschner, G., Balla, B., Horváth, P., Kövesdi, A., Lakatos, G., Takács, I., Nagy, Z., Tóbiás, B., Árvai, K., Pál Kósa, J., & Lakatos, P. (2016). Effects of imatinib and nilotinib on the whole transcriptome of cultured murine osteoblasts. Molecular Medicine Reports, 14(3), 2025-2037. https://doi.org/10.3892/mmr.2016.5459