Effects of Cu2+ and Pb2+ on different fish species: Liver cytochrome P450-dependent monooxygenase activities and FTIR spectra

Mária Henczová, Aranka Kiss Deér, Adrienn Filla, Viktória Komlósi, J. Mink

Research output: Article

16 Citations (Scopus)

Abstract

The effects of Cu2+-sulfate and Pb2+-acetate on carp (Cyprinus carpio L.), silver carp (Hypopthalmichtys molitrix V.) and wels (Silurus glanis L.) were studied. The liver microsomal Cyt P450 content, the EROD, ECOD and APND monooxygenase activities were measured. In vivo treatment with 1 mg L- 1 Cu2+ significantly elevated the activities of these enzymes and Cyt P450 content in silver carp livers. The high-dose Cu2+ treatment (10 mg L- 1) on silver carp caused two-fold higher induction in the P450 dependent monooxygenase isoensymes than in wels. Although the 2 mg kg- 1 treatment with Pb2+ in carp elevated significantly the P450 content, the EROD isoenzyme activities were significantly decreased after 1 day, showing the destructive effect of metal ion on the enzyme system. In vitro, Cu2+ and Pb2+ decreased the Cyt P450 content in the carp liver microsomes and the absorption peak shifted to higher wavelength. Fourier Transform Infrared (FTIR) spectroscopy was used to detect the damaging effects of the heavy metals. According to the inhibitory potency to Cu2+, the most sensitive isoenzyme was the EROD in wels, the least was the silver carp's isoenzyme. The investigated fish P450 isoenzymes showed, that the Cu2+ was a stronger inhibitor than Pb2+.

Original languageEnglish
Pages (from-to)53-60
Number of pages8
JournalComparative Biochemistry and Physiology - C Toxicology and Pharmacology
Volume148
Issue number1
DOIs
Publication statusPublished - júl. 2008

Fingerprint

Carps
Fourier Analysis
Mixed Function Oxygenases
Silver
Liver
Fish
Cytochrome P-450 Enzyme System
Isoenzymes
Cytochrome P-450 CYP1A1
Fourier transforms
Fishes
Infrared radiation
Enzymes
Heavy Metals
Sulfates
Metal ions
Acetates
Wavelength
Liver Microsomes
Fourier Transform Infrared Spectroscopy

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Health, Toxicology and Mutagenesis
  • Pharmacology

Cite this

Effects of Cu2+ and Pb2+ on different fish species : Liver cytochrome P450-dependent monooxygenase activities and FTIR spectra. / Henczová, Mária; Deér, Aranka Kiss; Filla, Adrienn; Komlósi, Viktória; Mink, J.

In: Comparative Biochemistry and Physiology - C Toxicology and Pharmacology, Vol. 148, No. 1, 07.2008, p. 53-60.

Research output: Article

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AB - The effects of Cu2+-sulfate and Pb2+-acetate on carp (Cyprinus carpio L.), silver carp (Hypopthalmichtys molitrix V.) and wels (Silurus glanis L.) were studied. The liver microsomal Cyt P450 content, the EROD, ECOD and APND monooxygenase activities were measured. In vivo treatment with 1 mg L- 1 Cu2+ significantly elevated the activities of these enzymes and Cyt P450 content in silver carp livers. The high-dose Cu2+ treatment (10 mg L- 1) on silver carp caused two-fold higher induction in the P450 dependent monooxygenase isoensymes than in wels. Although the 2 mg kg- 1 treatment with Pb2+ in carp elevated significantly the P450 content, the EROD isoenzyme activities were significantly decreased after 1 day, showing the destructive effect of metal ion on the enzyme system. In vitro, Cu2+ and Pb2+ decreased the Cyt P450 content in the carp liver microsomes and the absorption peak shifted to higher wavelength. Fourier Transform Infrared (FTIR) spectroscopy was used to detect the damaging effects of the heavy metals. According to the inhibitory potency to Cu2+, the most sensitive isoenzyme was the EROD in wels, the least was the silver carp's isoenzyme. The investigated fish P450 isoenzymes showed, that the Cu2+ was a stronger inhibitor than Pb2+.

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