Effects of benz/c/acridines and benzophenothiazines on testosterone secretion of leydig cell cultures of the rat

Z. Valkusz, M. Gálfi, A. Juhász, I. Tóth, F. Szarvas, Z. Hódi, J. Molnár, J. Julesz

Research output: Article

Abstract

Cell cultures were prepared from the testicles of 180 g bw. male Wistar rats separated on Percoll gradient. The cultures were treated with collagen in Petri dishes; they then were suspended in DMEM (+15% FCS, 100 U penicillin, +100 mg/ml streptomycin) medium (pCO2 5%; t=37°C). The density of the suspension was 105 Leydig cell/ml at start. The eight-day-old cultures were standardized immunohistochemically. These primary monolayer Leydig cell cultures were treated with the derivatives of Benz/c/acridines (BcA) and Benzophenothiazines (BPh) in doses 0,5 ug/ml, 2 ug/ml and 5 ug/ml. Leydig cells were specifically stimulated with 3 nM hCG for Testosteron (T) secretion. T was measured with RIA. All the investigated compounds proved to stimulate the basal T release, BcA-s were more effective in this regard. After hCG exposition the T level further increased. Since the chemical structures of BPh-s and BcA-s were different, we were able to establish a possible chemical structure-exocytotic activity relationship in our biological modell. Data obtained in this experiments might be useful in future chemotherapeutical application of these compounds. This work was supported by ETT grant No. T-11/592/93.

Original languageEnglish
Pages (from-to)S117
JournalMedical Microbiology Letters
Volume5
Issue numberSUPPL. 1
Publication statusPublished - dec. 1 1996

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ASJC Scopus subject areas

  • Microbiology (medical)

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