Cell cultures were prepared from the testicles of 180 g bw. male Wistar rats separated on Percoll gradient. The cultures were treated with collagen in Petri dishes; they then were suspended in DMEM (+15% FCS, 100 U penicillin, +100 mg/ml streptomycin) medium (pCO2 5%; t=37°C). The density of the suspension was 105 Leydig cell/ml at start. The eight-day-old cultures were standardized immunohistochemically. These primary monolayer Leydig cell cultures were treated with the derivatives of Benz/c/acridines (BcA) and Benzophenothiazines (BPh) in doses 0,5 ug/ml, 2 ug/ml and 5 ug/ml. Leydig cells were specifically stimulated with 3 nM hCG for Testosteron (T) secretion. T was measured with RIA. All the investigated compounds proved to stimulate the basal T release, BcA-s were more effective in this regard. After hCG exposition the T level further increased. Since the chemical structures of BPh-s and BcA-s were different, we were able to establish a possible chemical structure-exocytotic activity relationship in our biological modell. Data obtained in this experiments might be useful in future chemotherapeutical application of these compounds. This work was supported by ETT grant No. T-11/592/93.
|Journal||Medical Microbiology Letters|
|Issue number||SUPPL. 1|
|Publication status||Published - dec. 1 1996|
ASJC Scopus subject areas
- Microbiology (medical)