Effect of the characteristics of the phase system on the retention of proteins in hydrophobic interaction chromatography

László Szepesy, Géza Rippel

Research output: Article

17 Citations (Scopus)

Abstract

In contrast to reversed-phase chromatography (RPC), where the stationary phases available show only minor differences as regards the retention and selectivity of separation, in hydrophobic interaction chromatography (HIC) the various stationary phases may show different effects depending on the type of support and ligand applied. In this work, the data obtained on four commercially available HIC columns with five standard proteins using salts of different types were investigated. It was found that the slope-intercept plot derived from the ln k vs. salt molality relationship can furnish a useful characterization of the hydrophobic interaction chromatography of proteins. A new hydrophobicity index introduced in RPC can also be applied as an overall parameter to characterize hydrophobic interaction. By using the above parameters the effects of both the column type and salt can be evaluated. Characterization of the HIC columns can be of considerable assistance in the selection of the appropriate column for a given separation.

Original languageEnglish
Pages (from-to)337-344
Number of pages8
JournalJournal of Chromatography A
Volume668
Issue number2
DOIs
Publication statusPublished - máj. 13 1994

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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