Effect of streptozotocin-induced diabetes on kidney Na+/K+-ATPase

A. Vér, I. Szanto, P. Csermely, K. Kalff, E. Vegh, T. Banyasz, Z. Marcsek, T. Kovacs, J. Somogyi

Research output: Article

8 Citations (Scopus)

Abstract

The maximal capacity of low affinity ouabain binding sites in kidney medulla was found to be increased by 20 ± 3.8% after 2 weeks, and by 35 ± 4.5% in 4 weeks diabetes. However, in kidney cortex no similar changes could be detected. Westen blot analysis of Na+/K+-ATPase subunits in kidney medulla indicated a significant enhancement of both the α1 and β1 subunit in two and four weeks diabetic rats (α1: 35 ± 3.1, 51 ± 5.8% and β1: 31.3 ± 5.2 and 43.2 ± 6.8%, respectively). However, kidney cortex showed no significant change in any condition tested. In diabetes we could detect a significant change only in the medulla in case of the b subunit mRNA transcript, which showed 1.69 ± 0.59 and 2.89 ± 0.81 times increased in two and four weeks diabetic state, respectively. There was no change in the α1 subunit mRNA abundance. Insulin treatment of diabetic animals did not result in a complete reversal of diabetes-induced changes in ouabain binding capacity or in the amount of Na+/K+-ATPase α1 and β1 subunit protein and mRNA levels. Our data indicate a good correlation between changes in low affinity ouabain binding capacity and the level of α1 isoform in diabetic rats, and suggest an important role of the b subunit in the regulation of enzyme quantity.

Original languageEnglish
Pages (from-to)323-332
Number of pages10
JournalActa Physiologica Hungarica
Volume83
Issue number4
Publication statusPublished - 1995

Fingerprint

Experimental Diabetes Mellitus
Ouabain
Kidney Medulla
Kidney Cortex
Kidney
Messenger RNA
Protein Subunits
Protein Isoforms
Binding Sites
Insulin
Enzymes
sodium-translocating ATPase

ASJC Scopus subject areas

  • Physiology

Cite this

Vér, A., Szanto, I., Csermely, P., Kalff, K., Vegh, E., Banyasz, T., ... Somogyi, J. (1995). Effect of streptozotocin-induced diabetes on kidney Na+/K+-ATPase. Acta Physiologica Hungarica, 83(4), 323-332.

Effect of streptozotocin-induced diabetes on kidney Na+/K+-ATPase. / Vér, A.; Szanto, I.; Csermely, P.; Kalff, K.; Vegh, E.; Banyasz, T.; Marcsek, Z.; Kovacs, T.; Somogyi, J.

In: Acta Physiologica Hungarica, Vol. 83, No. 4, 1995, p. 323-332.

Research output: Article

Vér, A, Szanto, I, Csermely, P, Kalff, K, Vegh, E, Banyasz, T, Marcsek, Z, Kovacs, T & Somogyi, J 1995, 'Effect of streptozotocin-induced diabetes on kidney Na+/K+-ATPase', Acta Physiologica Hungarica, vol. 83, no. 4, pp. 323-332.
Vér, A. ; Szanto, I. ; Csermely, P. ; Kalff, K. ; Vegh, E. ; Banyasz, T. ; Marcsek, Z. ; Kovacs, T. ; Somogyi, J. / Effect of streptozotocin-induced diabetes on kidney Na+/K+-ATPase. In: Acta Physiologica Hungarica. 1995 ; Vol. 83, No. 4. pp. 323-332.
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