Effect of simulated microgravity on the production of IL-12 by PBMCs

Ágnes Bakos, Andrea Várkonyi, János Minárovits, László Bátkai

Research output: Conference article


During space flight immunity is altered. This phenomenon is partly due to the microgravity condition itself. Our earlier space experiments (INTERFERON) indicated that microgravity has a significant effect at the cellular level. In our subsequent terrestrial studies we applied the Rotating Cell Culture System (RCCS) developed by NASA to mimick microgravity on ground. Previously we reported that human peripheral blood mononuclear cells (PBMCs) respond to simulated microgravity conditions with elevated tumor necrosis factor-alpha (TNF-α) production. We extended our investigations to the production of interleukin (IL)-12 under modelled microgravity conditions by separated PBMCs. In simulated microgravity we found significantly elevated level of secreted IL-12 compared to static, standard tissue culture conditions. Following a maximum of TNF-α production at 24 hours, the peak of IL-12 production was observed at 48 hours after the start of the experiment. Our results suggest that simulated microgravity favors the establishment of a Th1 type cytokine response.

Original languageEnglish
Pages (from-to)335-336
Number of pages2
JournalEuropean Space Agency, (Special Publication) ESA SP
Issue number501
Publication statusPublished - szept. 1 2002
EventProceedings of the European Symposium on Life in Space for Life on Earth - Stockholm, Sweden
Duration: jún. 2 2002jún. 7 2002

ASJC Scopus subject areas

  • Aerospace Engineering
  • Space and Planetary Science

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