Effect of rabbit κ-casein expression on the properties of milk from transgenic mice

László Hiripi, Mária Baranyi, László Szabó, Szabolcs Tóth, Marie Louise Fontaine, Eve Devinoy, Z. Bösze

Research output: Article

11 Citations (Scopus)

Abstract

Transgenic mice were produced carrying the coding region of the rabbit κ-casein gene linked to the upstream region of the rabbit whey acidic protein gene. Mice from the highest-expressing line produced 2·5 mg rabbit κ-casein/ml in their milk. The foreign protein was associated with the casein micelles and altered micelle size, though in the high-expressing line rabbit κ-casein also segregated into the whey fraction obtained after centrifuging the milk samples. Milk from transgenic mice had the same overall protein content as that from non-transgenic mice, except for the transgene product. However, litters fed with this transgenic mouse milk grew less well than litters given milk from non-transgenic mice. This reduction in growth was not related to changes in mammary gland structure or mammary cell morphology. Preliminary results indicated that milk from the transgenic mice had a higher viscosity.

Original languageEnglish
Pages (from-to)541-550
Number of pages10
JournalJournal of Dairy Research
Volume67
Issue number4
DOIs
Publication statusPublished - 2000

Fingerprint

Caseins
Transgenic Mice
casein
Milk
rabbits
genetically modified organisms
Rabbits
milk
mice
Micelles
micelles
whey
Human Mammary Glands
Transgenes
Viscosity
Genes
mammary glands
Proteins
Breast
growth retardation

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Applied Microbiology and Biotechnology
  • Food Science

Cite this

Effect of rabbit κ-casein expression on the properties of milk from transgenic mice. / Hiripi, László; Baranyi, Mária; Szabó, László; Tóth, Szabolcs; Fontaine, Marie Louise; Devinoy, Eve; Bösze, Z.

In: Journal of Dairy Research, Vol. 67, No. 4, 2000, p. 541-550.

Research output: Article

Hiripi, L, Baranyi, M, Szabó, L, Tóth, S, Fontaine, ML, Devinoy, E & Bösze, Z 2000, 'Effect of rabbit κ-casein expression on the properties of milk from transgenic mice', Journal of Dairy Research, vol. 67, no. 4, pp. 541-550. https://doi.org/10.1017/S0022029900004386
Hiripi, László ; Baranyi, Mária ; Szabó, László ; Tóth, Szabolcs ; Fontaine, Marie Louise ; Devinoy, Eve ; Bösze, Z. / Effect of rabbit κ-casein expression on the properties of milk from transgenic mice. In: Journal of Dairy Research. 2000 ; Vol. 67, No. 4. pp. 541-550.
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