The pressure applied in liquid chromatography (LC) can influence the capacity factor of analytes. Using commercial equipment, column (33 x 4.6 mm) and 1.5 μm non-porous, tetradecyl (C14) coated silica particles, a moderate but significant change in the capacity factor was observed with increasing pressure. Depending on the experimental conditions the relationship was found to be either linear or non-linear. In the latter case the retention increased at first, but later tended to decrease at still higher pressure. The results direct attention to the role of the pressure (and hence of flow rate and heat of friction) in determining capacity factors.
|Publication status||Published - jan. 1 2000|
ASJC Scopus subject areas
- Analytical Chemistry
- Clinical Biochemistry
- Organic Chemistry