Effect of mobile phase composition on the liquid chromatographic enantioseparation of bulky monoterpene-based β-amino acids by applying chiral stationary phases based on Cinchona alkaloid

Zoltán Pataj, István Ilisz, Zsanett Gecse, Zsolt Szakonyi, Ferenc FüLöP, Wolfgang Lindner, Antal Péter

Research output: Article

23 Citations (Scopus)

Abstract

Stereoselective HPLC separations of five sterically constrained monoterpene-based 2-aminocarboxylic acid enantiomers were carried out by using the newly developed zwitterionic chiral stationary phases Chiralpak ZWIX(+)™ and ZWIX(-)™ based on Cinchona alkaloid. In order to optimize the retention and enantioselectivity parameters, the ratio of the different organic solvents in the mobile phase and the nature of the acid and base additives (counter- and co-ions) were systematically varied. The effects of structure variants of the analytes on the resolution were investigated. The elution sequence was determined in all cases and observed to be opposite on ZWIX(+)™ and ZWIX(-)™.

Original languageEnglish
Pages (from-to)1075-1082
Number of pages8
JournalJournal of separation science
Volume37
Issue number9-10
DOIs
Publication statusPublished - máj. 2014

ASJC Scopus subject areas

  • Analytical Chemistry
  • Filtration and Separation

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