Abstract
Aim: To study the expression of endothelial and inducible nitric oxide synthases (eNOS and iNOS) and their role in inflammatory bowel disease (IBD). Methods: We examined the effect of sera obtained from patients with active Crohn's disease (CD) and ulcerative colitis (UC) on the function and viability of human umbilical vein endothelial cells (HUVEC). HUVECs were cultured for 0-48 h in the presence of a medium containing pooled serum of healthy controls, or serum from patients with active CD or UC. Expression of eNOS and iNOS was visualized by immunofluorescence, and quantified by the densitometry of Western blots. Proliferation activity was assessed by computerized image analyses of Ki-67 immunoreactive cells, and also tested in the presence of the NOS inhibitor, 10-4 mol/ L L-NAME. Apoptosis and necrosis was examined by the annexin-V-biotin method and by propidium iodide staining, respectively. Results: In HUVE C immediately after exposure to UC, serum eNOS was markedly induced, reaching a peak at 12 h. In contrast, a decrease in eNOS was observed after incubation with CD sera and the eNOS level was minimal at 20 h compared to control (18%±16% vs 23%±15% P
| Original language | English |
|---|---|
| Pages (from-to) | 1730-1738 |
| Number of pages | 9 |
| Journal | World Journal of Gastroenterology |
| Volume | 12 |
| Issue number | 11 |
| Publication status | Published - márc. 21 2006 |
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ASJC Scopus subject areas
- Gastroenterology
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Effect of IBD sera on expression of inducible and endothelial nitric oxide synthase in human umbilical vein endothelial cells. / Palatka, K.; Serfőző, Z.; Veréb, Zoltán; Bátori, Róbert; Lontay, Beáta; Hargitay, Zoltán; Nemes, Z.; Urvardy, Miklós; Erdődi, F.; Altorjay, I.
In: World Journal of Gastroenterology, Vol. 12, No. 11, 21.03.2006, p. 1730-1738.Research output: Article
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TY - JOUR
T1 - Effect of IBD sera on expression of inducible and endothelial nitric oxide synthase in human umbilical vein endothelial cells
AU - Palatka, K.
AU - Serfőző, Z.
AU - Veréb, Zoltán
AU - Bátori, Róbert
AU - Lontay, Beáta
AU - Hargitay, Zoltán
AU - Nemes, Z.
AU - Urvardy, Miklós
AU - Erdődi, F.
AU - Altorjay, I.
PY - 2006/3/21
Y1 - 2006/3/21
N2 - Aim: To study the expression of endothelial and inducible nitric oxide synthases (eNOS and iNOS) and their role in inflammatory bowel disease (IBD). Methods: We examined the effect of sera obtained from patients with active Crohn's disease (CD) and ulcerative colitis (UC) on the function and viability of human umbilical vein endothelial cells (HUVEC). HUVECs were cultured for 0-48 h in the presence of a medium containing pooled serum of healthy controls, or serum from patients with active CD or UC. Expression of eNOS and iNOS was visualized by immunofluorescence, and quantified by the densitometry of Western blots. Proliferation activity was assessed by computerized image analyses of Ki-67 immunoreactive cells, and also tested in the presence of the NOS inhibitor, 10-4 mol/ L L-NAME. Apoptosis and necrosis was examined by the annexin-V-biotin method and by propidium iodide staining, respectively. Results: In HUVE C immediately after exposure to UC, serum eNOS was markedly induced, reaching a peak at 12 h. In contrast, a decrease in eNOS was observed after incubation with CD sera and the eNOS level was minimal at 20 h compared to control (18%±16% vs 23%±15% P
AB - Aim: To study the expression of endothelial and inducible nitric oxide synthases (eNOS and iNOS) and their role in inflammatory bowel disease (IBD). Methods: We examined the effect of sera obtained from patients with active Crohn's disease (CD) and ulcerative colitis (UC) on the function and viability of human umbilical vein endothelial cells (HUVEC). HUVECs were cultured for 0-48 h in the presence of a medium containing pooled serum of healthy controls, or serum from patients with active CD or UC. Expression of eNOS and iNOS was visualized by immunofluorescence, and quantified by the densitometry of Western blots. Proliferation activity was assessed by computerized image analyses of Ki-67 immunoreactive cells, and also tested in the presence of the NOS inhibitor, 10-4 mol/ L L-NAME. Apoptosis and necrosis was examined by the annexin-V-biotin method and by propidium iodide staining, respectively. Results: In HUVE C immediately after exposure to UC, serum eNOS was markedly induced, reaching a peak at 12 h. In contrast, a decrease in eNOS was observed after incubation with CD sera and the eNOS level was minimal at 20 h compared to control (18%±16% vs 23%±15% P
KW - Crohn's disease
KW - Human umbilical vein endothelial cells
KW - Inflammatory bowel disease
KW - Nitric oxide synthase
KW - Ulcerative colitis
UR - http://www.scopus.com/inward/record.url?scp=33645880135&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33645880135&partnerID=8YFLogxK
M3 - Article
C2 - 16586542
AN - SCOPUS:33645880135
VL - 12
SP - 1730
EP - 1738
JO - World Journal of Gastroenterology
JF - World Journal of Gastroenterology
SN - 1007-9327
IS - 11
ER -