A humán patogén Helicobacter pylori homogenizátum hatása patkány izolált gyomornyálkahártya sejtjeinek életképességére.

G. Mózsik, B. Bódis, O. Karádi, L. Nagy, S. Vörös

Research output: Article

Abstract

Aim of this study was to detect the possible direct cellular effect of Helicobacter pylori (Hp) (obtained by biopsy from patients with gastric and duodenal ulcer) on freshly isolated rat gastric mucosal cells. Urease test positive Hp cultured from human biopsy sample was sonicated and incubated with freshly isolated rat gastric mucosal cells. The direct cellular damaging effect of Hp was evaluated alone and in combination with ethanol and indomethacin. The cellular damaging effect was tested morphologically (Trypan blue exclusion) and biochemically (measurements of lactate dehydrogenase, succinic dehydrogenase and ethidium bromide DNA fluorescence). Hp alone, or in combination with ethanol and indomethacin had no direct cellular toxicity on freshly isolated rat gastric mucosal cells. The isolated and cultured Hp collected from human beings did not produce any cellular damage on freshly isolated rat gastric mucosal cells.

Original languageHungarian
Pages (from-to)1749-1752
Number of pages4
JournalOrvosi Hetilap
Volume137
Issue number32
Publication statusPublished - aug. 11 1996

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Helicobacter pylori
Stomach
Indomethacin
Ethanol
Biopsy
Ethidium
Succinate Dehydrogenase
Trypan Blue
Urease
Stomach Ulcer
Duodenal Ulcer
L-Lactate Dehydrogenase
Fluorescence
DNA

ASJC Scopus subject areas

  • Medicine(all)

Cite this

A humán patogén Helicobacter pylori homogenizátum hatása patkány izolált gyomornyálkahártya sejtjeinek életképességére. / Mózsik, G.; Bódis, B.; Karádi, O.; Nagy, L.; Vörös, S.

In: Orvosi Hetilap, Vol. 137, No. 32, 11.08.1996, p. 1749-1752.

Research output: Article

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