Effect of HGF-like basic hexapeptides on angiogenesis

K. Fazekas, A. Janovics, B. Döme, P. Koska, A. Albini, J. Tímár

Research output: Article

14 Citations (Scopus)

Abstract

The interaction of glycosaminoglycans (GAG) with peptides relies on noncovalent binding to basic amino acid sequences, for which a minimal requirement is a pentapeptide region in the protein and the sulfated and carboxyl region in the GAG. Since such sequences are present in the heparin-binding angiogenic cytokines, including hepatocyte growth factor (HGF), we have postulated that such small peptides may have biological activity. Two basic peptide regions of the β chain of HGF (RYRNKH512-516, HHRGK645-649) exhibited significant anti-angiogenic activity in viva in the chorioallantoic membrane assay and showed some antiproliferative activity in vitro on normal human brain microvessel endothelial - but not on anchorage-independent endothelial - cells (Kaposi sarcoma). Basic HIV-TAT peptides and scrambled hexapeptides did not show similar activity, except for KRKRKR, indicating sequence specificity of the phenomena. An HGF-derived basic peptide, HHRGK, modulated tumor-induced angiogenesis in viva by interfering with the morphogenic, but not with the proliferative, phase of the process. These observations suggest small basic peptides as a new class of angiogenesis modulators.

Original languageEnglish
Pages (from-to)440-444
Number of pages5
JournalMicrovascular Research
Volume62
Issue number3
DOIs
Publication statusPublished - 2001

Fingerprint

Hepatocyte Growth Factor
Peptides
Glycosaminoglycans
Angiogenesis Modulating Agents
Chorioallantoic Membrane
Basic Amino Acids
Kaposi's Sarcoma
Endothelial cells
Microvessels
Bioactivity
Heparin
Tumors
Amino Acid Sequence
Assays
Brain
Endothelial Cells
HIV
Cytokines
Membranes
Neoplasms

ASJC Scopus subject areas

  • Biochemistry
  • Cardiology and Cardiovascular Medicine

Cite this

Effect of HGF-like basic hexapeptides on angiogenesis. / Fazekas, K.; Janovics, A.; Döme, B.; Koska, P.; Albini, A.; Tímár, J.

In: Microvascular Research, Vol. 62, No. 3, 2001, p. 440-444.

Research output: Article

Fazekas, K. ; Janovics, A. ; Döme, B. ; Koska, P. ; Albini, A. ; Tímár, J. / Effect of HGF-like basic hexapeptides on angiogenesis. In: Microvascular Research. 2001 ; Vol. 62, No. 3. pp. 440-444.
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AU - Koska, P.

AU - Albini, A.

AU - Tímár, J.

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KW - Tumor-induced angiogenesis

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