Effect of frequently used chemotherapeutic drugs on cytotoxic activity of human cytotoxic T-lymphocytes

Laszlo Markasz, Henriette Skribek, Michael Uhlin, Rita Otvos, Emilie Flaberg, Staffan Eksborg, Eva Olah, Gyorgy Stuber, Laszlo Szekely

Research output: Article

25 Citations (Scopus)

Abstract

Tumors are considered to be possible targets of immunotherapy using stimulated and expanded cytotoxic T-lymphocytes (CTL). It is important to consider the drug-induced effects when chemotherapeutic regimens and CTL-mediated immunotherapy is planned to be used in parallel. In this study, we characterized the effect of 29 frequently used chemotherapeutic agents on the cytotoxic activity of autologous and allogeneic CTLs. We found that treatment of CTLs with the following drugs: docetaxel, vincristine, chlorambucil, mitomycin C, oxaliplatin, doxorubicin, and bleomycin effectively inhibited CTL-mediated killing, without affecting their viability. On the other hand, the following drugs enhanced or permitted efficient CTL-mediated killing in vitro at concentrations comparable with the maximally achieved therapeutic concentration in vivo in humans: daunorubicin, prednisolone, vinorelbine, cisplatin, methotrexate, hydroxyurea, cytarabine, cyclophosphamide, topotecan, epirubicin, fluorouracil, carboplatin, asparaginase, 6-mercaptopurine, and bortezomib. Our results could potentially be used in the future to design new CTL-based adjuvant immunotherapy protocols.

Original languageEnglish
Pages (from-to)283-293
Number of pages11
JournalJournal of Immunotherapy
Volume31
Issue number3
DOIs
Publication statusPublished - ápr. 1 2008

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Pharmacology
  • Cancer Research

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    Markasz, L., Skribek, H., Uhlin, M., Otvos, R., Flaberg, E., Eksborg, S., Olah, E., Stuber, G., & Szekely, L. (2008). Effect of frequently used chemotherapeutic drugs on cytotoxic activity of human cytotoxic T-lymphocytes. Journal of Immunotherapy, 31(3), 283-293. https://doi.org/10.1097/CJI.0b013e3181628b76