Effect of dipyridamole on transport and phosphorylation of thymidine and 3′-azido-3′-deoxythymidine in human monocyte/macrophages

Gurupadappa V. Betageri, Janos Szebeni, Kenneth Hung, Shaila S. Patel, Larry M. Wahl, Martha Corcoran, John N. Weinstein

Research output: Article

12 Citations (Scopus)

Abstract

Dipyridamole (DPM), a commonly used coronary vasodilator and antithrombotic drug, was shown recently to potentiate the antiviral effect of 3′-azido-3′-deoxythymidine (AZT) in HIV-1 infected human monocyte-derived macrophages (M/M) in vitro. We report in the present study that in uninfected M/M, DPM markedly inhibited cellular uptake of [3H]thymidine (dThd) and its incorporation into the nucleotide pools, particularly the dThd-triphosphate pool. In contrast, DPM did not affect cellular uptake and phosphorylation of [3H]AZT. Since dThd counteracts the phosphorylation and antiviral action of AZT, these findings support the hypothesis that the potentiation of the anti-HIV effect of AZT is due, at least in part, to differential inhibition of nucleoside salvage.

Original languageEnglish
Pages (from-to)867-870
Number of pages4
JournalBiochemical Pharmacology
Volume40
Issue number4
DOIs
Publication statusPublished - aug. 15 1990

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ASJC Scopus subject areas

  • Biochemistry
  • Pharmacology

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