Effect of α-methyldopa on pentoxyresorufin O-dealkylation in liver microsomes from rats treated with phenobarbital

Z. Veres, T. Szakács, L. Vereczkey

Research output: Article


Loss of pentoxyresorufin O-dealkylation (PROD) was observed when microsomes from PB-treated rats were preincubated in the presence of NADPH. PROD proved to be quite sensitive towards inactivation. Decrease in cytochrome P450 (CYP) dependent activity was accompanied by simultaneous formation of thiobarbituric acid reactive substances (TBARS) indicating the occurrence of lipid peroxidation. The presence of 50 μM α-methyldopa (AMD) during preincubation with NADPH resulted in complete protection against enzyme activity loss and the extent of lipid peroxidation was also diminished. Addition of ascorbate or GSH in combination with AMD reduced the protective effect of the drug on PROD. AMD probably exerts its effect by scavenging reactive oxygen species but chelation of ferric ions can also contribute to the protective effect of the drug on PROD activity.

Original languageEnglish
Pages (from-to)59-64
Number of pages6
JournalArchives of Biochemistry and Biophysics
Issue number1
Publication statusPublished - aug. 1 2001


ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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