DNA adducts in peripheral blood lymphocytes from aluminum production plant workers determined by 32P- postlabeling and enzyme-linked immunosorbent assay

B. Schoket, D. H. Phillips, M. C. Poirier, I. Vincze

Research output: Article

32 Citations (Scopus)

Abstract

32P-Postlabeling analysis and enzyme-linked immunosorbent assay (ELISA) have been used to detect DNA adducts in peripheral blood lymphocytes from primary aluminum production plant workers who were exposed occupationally to a mixture of polycyclic aromatic hydrocarbons (PAHs). Preliminary results reported here are from a comparative study being performed in two aluminum plants. The levels of aromatic DNA adducts have been determined by the 32P-postlabeling assay in samples collected on two occasions, 1 year apart. PAH-DNA adduct levels have also been determined by competitive ELISA in the second set of DNA samples. The results show the necessity of follow-up biomonitoring studies to detect possible alterations in biological effect induced by changing exposures. The comparison of the results obtained by 32P-postlabeling and ELISA may lead to a better understanding of the power and weaknesses of the two methods applied in these studies.

Original languageEnglish
Pages (from-to)307-309
Number of pages3
JournalEnvironmental Health Perspectives
Volume99
Publication statusPublished - 1993

Fingerprint

Immunosorbents
Lymphocytes
DNA Adducts
Aluminum
Assays
Blood
aluminum
blood
Enzyme-Linked Immunosorbent Assay
assay
enzyme
DNA
Enzymes
PAH
Aluminum plants
Environmental Monitoring
Polycyclic Aromatic Hydrocarbons
biomonitoring
comparative study
plant production

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Public Health, Environmental and Occupational Health

Cite this

@article{11a33501930c4f498f108c86bbc91d18,
title = "DNA adducts in peripheral blood lymphocytes from aluminum production plant workers determined by 32P- postlabeling and enzyme-linked immunosorbent assay",
abstract = "32P-Postlabeling analysis and enzyme-linked immunosorbent assay (ELISA) have been used to detect DNA adducts in peripheral blood lymphocytes from primary aluminum production plant workers who were exposed occupationally to a mixture of polycyclic aromatic hydrocarbons (PAHs). Preliminary results reported here are from a comparative study being performed in two aluminum plants. The levels of aromatic DNA adducts have been determined by the 32P-postlabeling assay in samples collected on two occasions, 1 year apart. PAH-DNA adduct levels have also been determined by competitive ELISA in the second set of DNA samples. The results show the necessity of follow-up biomonitoring studies to detect possible alterations in biological effect induced by changing exposures. The comparison of the results obtained by 32P-postlabeling and ELISA may lead to a better understanding of the power and weaknesses of the two methods applied in these studies.",
author = "B. Schoket and Phillips, {D. H.} and Poirier, {M. C.} and I. Vincze",
year = "1993",
language = "English",
volume = "99",
pages = "307--309",
journal = "Environmental Health Perspectives",
issn = "0091-6765",
publisher = "Public Health Services, US Dept of Health and Human Services",

}

TY - JOUR

T1 - DNA adducts in peripheral blood lymphocytes from aluminum production plant workers determined by 32P- postlabeling and enzyme-linked immunosorbent assay

AU - Schoket, B.

AU - Phillips, D. H.

AU - Poirier, M. C.

AU - Vincze, I.

PY - 1993

Y1 - 1993

N2 - 32P-Postlabeling analysis and enzyme-linked immunosorbent assay (ELISA) have been used to detect DNA adducts in peripheral blood lymphocytes from primary aluminum production plant workers who were exposed occupationally to a mixture of polycyclic aromatic hydrocarbons (PAHs). Preliminary results reported here are from a comparative study being performed in two aluminum plants. The levels of aromatic DNA adducts have been determined by the 32P-postlabeling assay in samples collected on two occasions, 1 year apart. PAH-DNA adduct levels have also been determined by competitive ELISA in the second set of DNA samples. The results show the necessity of follow-up biomonitoring studies to detect possible alterations in biological effect induced by changing exposures. The comparison of the results obtained by 32P-postlabeling and ELISA may lead to a better understanding of the power and weaknesses of the two methods applied in these studies.

AB - 32P-Postlabeling analysis and enzyme-linked immunosorbent assay (ELISA) have been used to detect DNA adducts in peripheral blood lymphocytes from primary aluminum production plant workers who were exposed occupationally to a mixture of polycyclic aromatic hydrocarbons (PAHs). Preliminary results reported here are from a comparative study being performed in two aluminum plants. The levels of aromatic DNA adducts have been determined by the 32P-postlabeling assay in samples collected on two occasions, 1 year apart. PAH-DNA adduct levels have also been determined by competitive ELISA in the second set of DNA samples. The results show the necessity of follow-up biomonitoring studies to detect possible alterations in biological effect induced by changing exposures. The comparison of the results obtained by 32P-postlabeling and ELISA may lead to a better understanding of the power and weaknesses of the two methods applied in these studies.

UR - http://www.scopus.com/inward/record.url?scp=0027192093&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027192093&partnerID=8YFLogxK

M3 - Article

C2 - 8319650

AN - SCOPUS:0027192093

VL - 99

SP - 307

EP - 309

JO - Environmental Health Perspectives

JF - Environmental Health Perspectives

SN - 0091-6765

ER -