In addition to the morphological criteria traditionally used in taxonomy, molecular markers, which could be useful as DNA barcodes, are of increasing interest for the identification of fungi. The success of unequivocal identification relies on a rational selection of potential marker genes. The search for a candidate that is universally applicable for fungal identification is currently the subject of much debate. The aim of this study is to investigate the reliability of genes from the ribosomal RNA cluster, small subunit (SSU) 18S and large subunit (LSU) 28S ribosomal DNA (rDNA), internal transcribed spacer (ITS) 1 and 2 interrupted by 5.8S rDNA as identification markers. For this purpose a total of 98 fungal strains isolated from different habitats in diverse geographic regions in the northern hemisphere were examined. The fungi investigated comprise 68 mucoralean, two mortierellalean and one kickxellalean zygomycete, as well as several ascomycetes and basidiomycetes, which serve as outgroups for the comparison. Phylogenetic reconstruction and analysis of a dataset encompassing a total of 115 new sequences (Acc nos. EU484190-EU484303, EF589886) including ITS1-5.8S-ITS2 nucleotide sequences of morphologically well-defined type strains suggest that the accessibility of type strains which can serve as references are an essential prerequisite for obtaining reliable results.
|Title of host publication||Current Advances in Molecular Mycology|
|Publisher||Nova Science Publishers, Inc.|
|Number of pages||50|
|Publication status||Published - dec. 1 2008|
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Biochemistry, Genetics and Molecular Biology(all)