Direct detection of free radicals and reactive oxygen species in thylakoids.

Research output: Article

12 Citations (Scopus)


In plants, reactive oxygen species (ROS), also known as active oxygen species (AOS), are associated with normal, physiologic processes as well as with responses to adverse conditions. ROS are connected to stress in many ways: as primary elicitors, as products and propagators of oxidative damage, or as signal molecules initiating defense or adaptation. The photosynthetic electron transport is a major site of oxidative stress by visible or ultraviolet light, high or low temperature, pollutants or herbicides. ROS production can be presumed from detecting oxidatively damaged lipids, proteins, or pigments as well as from the alleviating effects of added antioxidants. On the contrary, measuring ROS by special sensor molecules provides more direct information. This chapter focuses on the application of spin trapping electron paramagnetic resonance (EPR) spectroscopy for detecting ROS: singlet oxygen and oxygen free radicals in thylakoid membrane preparations.

Original languageEnglish
Pages (from-to)187-200
Number of pages14
JournalMethods in molecular biology (Clifton, N.J.)
Publication statusPublished - 2011

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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