Differentiation-regulated expression of toll-like receptors 2 and 4 in HaCat keratinocytes

Andor Pivarcsi, Andrea Koreck, Laszlo Bodai, Márta Széll, Csilla Szeg, Nóra Belső, Anna Kenderessy-Szabó, Zsuzsanna Bata-Csörgő, Attila Dobozy, Lajos Kemény

Research output: Article

38 Citations (Scopus)


Toll-like receptors (TLRs) play an important role in the recognition of pathogens in keratinocytes. In this study, we investigated whether the differentiation state of HaCaT keratinocytes correlates with the expression of TLR2 and TLR4 genes. The expression levels of TLR2 and TLR4 in a HaCaT differentiation model system were determined using quantitative real-time RT-PCR (Q-RT-PCR) and flow cytometry. The progression of keratinocyte differentiation was monitored by determining the level of involucrin gene expression using Q-RT-PCR. The expression levels of TLR2 and TLR4 increased with the stage of differentiation and there were strong correlations between the expression level of the involucrin gene and those of the TLR2 gene (r=0.809, P<0.0001) and the TLR4 gene (r=0.568, P<0.02). Increased cell surface expression of TLR2 and TLR4 was also found in differentiated HaCaT keratinocytes by flow cytometric analysis. Our findings suggest that upregulation of TLR expression during differentiation in keratinocytes could be a part of the differentiation process of keratinocytes and could have biological significance in protecting skin against microbes.

Original languageEnglish
Pages (from-to)120-124
Number of pages5
JournalArchives of Dermatological Research
Issue number3
Publication statusPublished - aug. 2004


ASJC Scopus subject areas

  • Dermatology

Cite this