Differential dimer activities of the transcription factor Oct-1 by DNA-induced interface swapping

A. Reményi, Alexey Tomilin, Ehmke Pohl, Katharina Lins, Ansgar Philippsen, Rolland Reinbold, Hans R. Schöler, Matthias Wilmanns

Research output: Article

91 Citations (Scopus)

Abstract

Two crystal structures of Oct-1 POU domain bound to DNA provide a rationale for differential, conformation-dependent recruitment of transcription cofactors. The POU-homeo and POU-specific subdomains of Oct-1 contain two different nonoverlapping pairs of surface patches that are capable of forming unrelated protein-protein interfaces. Members of the POU factor family contain one or two conserved sequence motifs in the interface that are known to be phosphorylated, as noted for Oct-1 and Pit-1. Modeling of Oct-4 reveals the unique case where the same conserved sequence is located in both interfaces. Our studies provide the basis for two distinct dimeric POU factor arrangements that are dictated by the architecture of each DNA response element. We suggest interface swapping in dimers could be a general mechanism of modulating the activity of transcription factors.

Original languageEnglish
Pages (from-to)569-580
Number of pages12
JournalMolecular Cell
Volume8
Issue number3
DOIs
Publication statusPublished - 2001

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Octamer Transcription Factor-1
Conserved Sequence
DNA
Response Elements
Proteins
Transcription Factors

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Differential dimer activities of the transcription factor Oct-1 by DNA-induced interface swapping. / Reményi, A.; Tomilin, Alexey; Pohl, Ehmke; Lins, Katharina; Philippsen, Ansgar; Reinbold, Rolland; Schöler, Hans R.; Wilmanns, Matthias.

In: Molecular Cell, Vol. 8, No. 3, 2001, p. 569-580.

Research output: Article

Reményi, A, Tomilin, A, Pohl, E, Lins, K, Philippsen, A, Reinbold, R, Schöler, HR & Wilmanns, M 2001, 'Differential dimer activities of the transcription factor Oct-1 by DNA-induced interface swapping', Molecular Cell, vol. 8, no. 3, pp. 569-580. https://doi.org/10.1016/S1097-2765(01)00336-7
Reményi, A. ; Tomilin, Alexey ; Pohl, Ehmke ; Lins, Katharina ; Philippsen, Ansgar ; Reinbold, Rolland ; Schöler, Hans R. ; Wilmanns, Matthias. / Differential dimer activities of the transcription factor Oct-1 by DNA-induced interface swapping. In: Molecular Cell. 2001 ; Vol. 8, No. 3. pp. 569-580.
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