Dietary energy substrates reverse early neuronal hyperactivity in a mouse model of Alzheimer's disease

Misha Zilberter, Anton Ivanov, Sofya Ziyatdinova, Marat Mukhtarov, Anton Malkov, Alan Alpár, Giuseppe Tortoriello, Catherine H. Botting, Lívia Fülöp, Alex A. Osypov, Asla Pitkänen, Heikki Tanila, Tibor Harkany, Yuri Zilberter

Research output: Article

38 Citations (Scopus)

Abstract

Deficient energy metabolism and network hyperactivity are the early symptoms of Alzheimer's disease (AD). In this study, we show that administration of exogenous oxidative energy substrates (OES) corrects neuronal energy supply deficiency that reduces the amyloid-beta-induced abnormal neuronal activity in vitro and the epileptic phenotype in AD model in vivo. In vitro, acute application of protofibrillar amyloid-β1-42 (Aβ 1-42) induced aberrant network activity in wild-type hippocampal slices that was underlain by depolarization of both the neuronal resting membrane potential and GABA-mediated current reversal potential. Aβ1-42 also impaired synaptic function and long-term potentiation. These changes were paralleled by clear indications of impaired energy metabolism, as indicated by abnormal NAD(P)H signaling induced by network activity. However, when glucose was supplemented with OES pyruvate and 3-beta-hydroxybutyrate, Aβ1-42 failed to induce detrimental changes in any of the above parameters. We administered the same OES as chronic supplementation to a standard diet to APPswe/PS1dE9 transgenic mice displaying AD-related epilepsy phenotype. In the ex-vivo slices, we found neuronal subpopulations with significantly depolarized resting and GABA-mediated current reversal potentials, mirroring abnormalities we observed under acute Aβ1-42 application. Ex-vivo cortex of transgenic mice fed with standard diet displayed signs of impaired energy metabolism, such as abnormal NAD(P)H signaling and strongly reduced tolerance to hypoglycemia. Transgenic mice also possessed brain glycogen levels twofold lower than those of wild-type mice. However, none of the above neuronal and metabolic dysfunctions were observed in transgenic mice fed with the OES-enriched diet. In vivo, dietary OES supplementation abated neuronal hyperexcitability, as the frequency of both epileptiform discharges and spikes was strongly decreased in the APPswe/PS1dE9 mice placed on the diet. Altogether, our results suggest that early AD-related neuronal malfunctions underlying hyperexcitability and energy metabolism deficiency can be prevented by dietary supplementation with native energy substrates. Read the Editorial Highlight for this article on doi: 10.1111/jnc.12138. When Alzheimer's disease is diagnosed, it is too late for any known treatment. Earlier stages are characterized by metabolic crisis and neuronal hyperactivity leading to increased energy demands, thus creating a vicious circle. We show that dietary supplementation with natural energy substrates in Alzheimer's mice model breaks this vicious circle reversing epileptogenesis in vivo and correcting neuronal pathologies ex vivo/in vitro.

Original languageEnglish
Pages (from-to)157-171
Number of pages15
JournalJournal of neurochemistry
Volume125
Issue number1
DOIs
Publication statusPublished - ápr. 1 2013

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ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Zilberter, M., Ivanov, A., Ziyatdinova, S., Mukhtarov, M., Malkov, A., Alpár, A., Tortoriello, G., Botting, C. H., Fülöp, L., Osypov, A. A., Pitkänen, A., Tanila, H., Harkany, T., & Zilberter, Y. (2013). Dietary energy substrates reverse early neuronal hyperactivity in a mouse model of Alzheimer's disease. Journal of neurochemistry, 125(1), 157-171. https://doi.org/10.1111/jnc.12127